在基于细胞的检测中，干扰素-β和干扰素-γ是埃博拉病毒的弱抑制剂。
Interferon-β and Interferon-γ Are Weak Inhibitors of Ebola Virus in Cell-Based Assays.
作者信息
Dyall Julie, Hart Brit J, Postnikova Elena, Cong Yu, Zhou Huanying, Gerhardt Dawn M, Freeburger Denise, Michelotti Julia, Honko Anna N, DeWald Lisa Evans, Bennett Richard S, Olinger Gene G, Jahrling Peter B, Hensley Lisa E
机构信息
Integrated Research Facility and.
Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland.
出版信息
J Infect Dis. 2017 May 1;215(9):1416-1420. doi: 10.1093/infdis/jix134.
Abstract
Previous studies have demonstrated little efficacy of interferons (IFNs) in animal models of Ebola virus disease. However, these studies were limited to a small number of type I IFNs and, during the most recent outbreak of Ebola virus, questions regarding the suitability of the animal models to evaluate IFNs were raised. To address the potential that anti-Ebola virus activity was overlooked, type I and type II IFNs (α-2a, α-2b, -β, -γ, and -universal) were tested in a variety of cell types (Vero E6, Huh 7 cells, and human macrophages). IFNs are weak inhibitors of Ebola virus Makona in these cell lines.
摘要
先前的研究表明，干扰素（IFNs）在埃博拉病毒病动物模型中的疗效甚微。然而，这些研究仅限于少数几种I型干扰素，并且在最近一次埃博拉病毒爆发期间，人们对用于评估干扰素的动物模型的适用性提出了质疑。为了探究抗埃博拉病毒活性被忽视的可能性，研究人员在多种细胞类型（Vero E6细胞、Huh 7细胞和人类巨噬细胞）中测试了I型和II型干扰素（α-2a、α-2b、β、γ和通用型）。在这些细胞系中，干扰素是埃博拉病毒马科纳株的弱抑制剂。
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