Kamp F, Astumian R D, Westerhoff H V
National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD 20892.
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3792-6. doi: 10.1073/pnas.85.11.3792.
For a transmembrane redox enzyme and a (passive) protonophore, the complete set of rate equations is given. Turnover causes cyclic variation of their electric polarization. This is responsible not only for effects of the electric field on the rate constants but also for the generation of an electric field felt by neighboring molecules. It is calculated that, when the systems are close together at a fixed distance, cycling of the two systems becomes coupled enabling the protonophore to pump protons against their electrochemical gradient. If the electrochemical gradient for protons approaches the input force of the redox reaction, slip (incomplete coupling between the chemical and proton-transport reactions) results. By using different sets of parameters, both kinetically reversible and kinetically irreversible proton pumps can be simulated.
对于一种跨膜氧化还原酶和一种（被动）质子载体，给出了完整的速率方程组。周转导致它们的电极化呈周期性变化。这不仅导致电场对速率常数产生影响，还会产生相邻分子所感受到的电场。据计算，当两个系统在固定距离处靠得很近时，两个系统的循环会相互耦合，使质子载体能够逆着其电化学梯度泵送质子。如果质子的电化学梯度接近氧化还原反应的输入力，就会出现滑脱（化学和质子传输反应之间的不完全耦合）。通过使用不同的参数集，可以模拟动力学可逆和动力学不可逆的质子泵。
