Ishida Momoko, Nishi Kosuke, Kunihiro Nanami, Onda Hiroyuki, Nishimoto Sogo, Sugahara Takuya
Faculty of Agriculture, Ehime University, Matsuyama, Ehime, Japan.
Food and Health Sciences Research Center, Ehime University, Matsuyama, Ehime, Japan.
J Sci Food Agric. 2017 Nov;97(14):4727-4736. doi: 10.1002/jsfa.8341. Epub 2017 May 3.
Coriandrum sativum L. seed is generally used as a spice and crude drug. Although many functions of the various components in C. sativum L. seed have been reported, the immunostimulatory effect of water-soluble components in C. sativum L. seed has not been studied. In the present study, we focused on the immunostimulatory effect of C. sativum L. seed aqueous extract (CAE) on macrophages as a novel health function of C. sativum L. seed components.
CAE significantly enhanced the production of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in both RAW264.7 cells and peritoneal macrophages by enhancing the expression levels of these cytokine genes. CAE also stimulated nitric oxide (NO) production and the phagocytosis activity in RAW264.7 cells. We suggest that the activity of CAE is a result of the upregulation of mitogen-activated protein kinase and nuclear factor-κB cascades via TLR4. In addition, IL-6 production by peritoneal macrophages collected from CAE-administered mice was significantly enhanced, suggesting that CAE could stimulate macrophage activity in vivo.
The findings of the present study suggest that CAE contains a novel water-soluble component with an immunostimulatory effect on macrophages. CAE would contribute to activating host defense against pathogens by stimulating the innate immunity. © 2017 Society of Chemical Industry.
芫荽籽通常用作香料和天然药物。尽管芫荽籽中各种成分的许多功能已有报道，但芫荽籽中水溶性成分的免疫刺激作用尚未得到研究。在本研究中，我们关注芫荽籽水提取物（CAE）对巨噬细胞的免疫刺激作用，将其作为芫荽籽成分的一种新的健康功能。
CAE通过提高这些细胞因子基因的表达水平，显著增强了RAW264.7细胞和腹腔巨噬细胞中肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的产生。CAE还刺激了RAW264.7细胞中一氧化氮（NO）的产生和吞噬活性。我们认为，CAE的活性是通过TLR4上调丝裂原活化蛋白激酶和核因子-κB级联反应的结果。此外，从给予CAE的小鼠收集的腹腔巨噬细胞产生的IL-6显著增强，表明CAE可以在体内刺激巨噬细胞活性。
本研究结果表明，CAE含有一种对巨噬细胞具有免疫刺激作用的新型水溶性成分。CAE通过刺激先天免疫，有助于激活宿主对病原体的防御。©2017化学工业协会。
