Department of Pediatric Surgery, Chung Shan Medical University Hospital, Taichung, Taiwan.
J Sci Food Agric. 2010 Aug 30;90(11):1846-54. doi: 10.1002/jsfa.4023.
Coriandrum sativum is used not only as a spice to aid flavour and taste values in food, but also as a folk medicine in many countries. Since little is known about the anti-inflammatory ability of the aerial parts (stem and leaf) of C. sativum, the present study investigated the effect of aerial parts of C. sativum on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. We further explored the molecular mechanism underlying these pharmacological properties of C. sativum.
Ethanolic extracts from both stem and leaf of C. sativum (CSEE) significantly decreased LPS-induced nitric oxide and prostaglandin E(2) production as well as inducible nitric oxide synthase, cyclooxygenase-2, and pro-interleukin-1beta expression. Moreover, LPS-induced IkappaB-alpha phosphorylation and nuclear p65 protein expression as well as nuclear factor-kappaB (NF-kappaB) nuclear protein-DNA binding affinity and reporter gene activity were dramatically inhibited by aerial parts of CSEE. Exogenous addition of CSEE stem and leaf significantly reduced LPS-induced expression of phosphorylated mitogen-activated protein kinases (MAPKs).
Our data demonstrated that aerial parts of CSEE have a strong anti-inflammatory property which inhibits pro-inflammatory mediator expression by suppressing NF-kappaB activation and MAPK signal transduction pathway in LPS-induced macrophages.
芫荽不仅作为一种香料，可增加食物的风味和口感，而且在许多国家也被用作民间药物。由于人们对芫荽（Coriandrum sativum）地上部分（茎和叶）的抗炎能力知之甚少，本研究调查了芫荽地上部分对脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞的影响。我们进一步探讨了芫荽地上部分发挥这些药理作用的分子机制。
芫荽的茎叶乙醇提取物（CSEE）可显著降低 LPS 诱导的一氧化氮和前列腺素 E2 的产生，以及诱导型一氧化氮合酶、环氧化酶-2 和促白细胞介素-1β的表达。此外，CSEE 还可显著抑制 LPS 诱导的 IkappaB-α磷酸化和核 p65 蛋白表达，以及核因子-κB（NF-κB）核蛋白-DNA 结合亲和力和报告基因活性。外源性添加 CSEE 茎叶可显著降低 LPS 诱导的磷酸化丝裂原活化蛋白激酶（MAPKs）的表达。
我们的数据表明，CSEE 的地上部分具有很强的抗炎特性，可通过抑制 NF-κB 激活和 LPS 诱导的巨噬细胞中 MAPK 信号转导通路来抑制促炎介质的表达。
