Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Plant Biotechnol J. 2017 Dec;15(12):1533-1543. doi: 10.1111/pbi.12735. Epub 2017 May 12.
ADP-glucose pyrophosphorylase, comprising two small subunits and two large subunits, is considered a key enzyme in the endosperm starch synthesis pathway in wheat (Triticum aestivum L.). Two genes, TaAGP-S1-7A and TaAGP-L-1B, were investigated in this study. Haplotypes of these genes were associated with thousand kernel weight (TKW) in different populations. Mean TKWs of favoured haplotypes were significantly higher than those of nonfavoured ones. Two molecular markers developed to distinguish these haplotypes could be used in molecular breeding. Frequencies of favoured haplotypes were dramatically increased in cultivars released in China after the 1940s. These favoured haplotypes were also positively selected in six major wheat production regions globally. Selection of AGP-S1 and AGP-L-1B in wheat mainly occurred during and after hexaploidization. Strong additive effects of the favoured haplotypes of with other genes for starch synthesis were also detected in different populations.
ADP-葡萄糖焦磷酸化酶由两个小亚基和两个大亚基组成，被认为是小麦（Triticum aestivum L.）胚乳淀粉合成途径中的关键酶。本研究对两个基因 TaAGP-S1-7A 和 TaAGP-L-1B 进行了研究。这些基因的单倍型与不同群体的千粒重（TKW）有关。有利单倍型的平均 TKW 明显高于不利单倍型。开发的两个区分这些单倍型的分子标记可用于分子育种。20 世纪 40 年代以后，中国育成的品种中有利单倍型的频率显著增加。这些有利单倍型在全球六个主要小麦生产地区也受到了积极选择。AGP-S1 和 AGP-L-1B 在小麦中的选择主要发生在六倍体化期间和之后。在不同群体中还检测到与其他淀粉合成基因有利单倍型的强加性效应。
