重组三段式皮钦德病毒作为一种新型活病毒疫苗平台。
Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform.
作者信息
Dhanwani Rekha, Ly Hinh, Liang Yuying
机构信息
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1988 Fitch Ave., Ste 295, Animal Science/Veterinary Medicine Building, Saint Paul, MN, 55108, USA.
La Jolla Institute for Allergy & Immunology, 9420 Athena Circle, La Jolla, CA, 92037, USA.
出版信息
Methods Mol Biol. 2017;1581:169-179. doi: 10.1007/978-1-4939-6869-5_10.
Abstract
Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired.
摘要
皮钦德病毒（PICV）是一种非致病性沙粒病毒，其RNA基因组由两个片段（L和S片段）组成，编码四种病毒基因。我们开发了一种反向遗传学系统，以产生重组三段式PICV（rP18tri），该病毒包装三个RNA片段（L、S1和S2），最多可编码两个外源基因。以流感病毒HA和NP作为模型抗原，我们发现rP18tri载体可诱导强烈的体液免疫和细胞介导免疫，加强免疫后免疫反应进一步增强。我们认为，这种新型rP18tri载体可开发成为针对其他抗原的有效疫苗平台，特别是在需要强大细胞免疫和初免-加强免疫策略的情况下。
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