Gultekin Mehmet, Ural Kerem, Aysul Nuran, Ayan Adnan, Balikci Canberk, Akyildiz Gurkan
a Faculty of Veterinary, Department of Internal Medicine , Adnan Menderes University , Isikli , Turkey.
b Faculty of Veterinary, Department of Parasitology , Adnan Menderes University , Isikli , Turkey.
Int J Environ Health Res. 2017 Jun;27(3):161-168. doi: 10.1080/09603123.2017.1310187. Epub 2017 Apr 4.
The purpose of the present study was to investigate the prevalence and molecular characterization of Giardia duodenalis among dogs in Aydin, Turkey. A total of 473 faecal samples from dogs were collected. The overall prevalence of G. duodenalis was 18.8%. Higher infection rates were observed in dogs younger than three months old, from shelters, and with diarrhoea. Faecal samples of 89 dogs, diagnosed Giardia-positive by microscopy, were found positive by nested PCR. The β-giardin nested PCR assay revealed assemblage B in all samples (100%), whereas 38 of the samples were mixed with assemblage A (42%). Sequence analysis of isolates indicated sub-genotypes A3 and B4 which have been previously detected in human isolates from Turkey. The results of the present study indicated the relatively high prevalence of giardiasis and the presence of the zoonotic sub-genotypes suggesting the important role of dogs as potential reservoirs of human infections.
本研究的目的是调查土耳其艾登省犬类中十二指肠贾第虫的流行情况及其分子特征。共收集了473份犬类粪便样本。十二指肠贾第虫的总体流行率为18.8%。在三个月龄以下的犬、来自收容所的犬以及患有腹泻的犬中观察到较高的感染率。通过显微镜诊断为贾第虫阳性的89只犬的粪便样本，经巢式PCR检测也呈阳性。β-贾第虫巢式PCR检测显示所有样本（100%）均为B群，而其中38个样本与A群混合（42%）。分离株的序列分析表明为A3和B4亚基因型，此前在土耳其的人类分离株中已检测到。本研究结果表明贾第虫病的流行率相对较高，且存在人畜共患亚基因型，提示犬作为人类感染潜在储存宿主的重要作用。
