Yoshinobu Tatsuo, Miyamoto Ko-Ichiro, Werner Carl Frederik, Poghossian Arshak, Wagner Torsten, Schöning Michael J
Department of Biomedical Engineering, Tohoku University, Sendai 980-8579, Japan; email:
Department of Electronic Engineering, Tohoku University, Sendai 980-8579, Japan.
Annu Rev Anal Chem (Palo Alto Calif). 2017 Jun 12;10(1):225-246. doi: 10.1146/annurev-anchem-061516-045158. Epub 2017 Mar 27.
A light-addressable potentiometric sensor (LAPS) is a semiconductor-based chemical sensor, in which a measurement site on the sensing surface is defined by illumination. This light addressability can be applied to visualize the spatial distribution of pH or the concentration of a specific chemical species, with potential applications in the fields of chemistry, materials science, biology, and medicine. In this review, the features of this chemical imaging sensor technology are compared with those of other technologies. Instrumentation, principles of operation, and various measurement modes of chemical imaging sensor systems are described. The review discusses and summarizes state-of-the-art technologies, especially with regard to the spatial resolution and measurement speed; for example, a high spatial resolution in a submicron range and a readout speed in the range of several tens of thousands of pixels per second have been achieved with the LAPS. The possibility of combining this technology with microfluidic devices and other potential future developments are discussed.
光寻址电位传感器（LAPS）是一种基于半导体的化学传感器，其中传感表面上的测量位点由光照定义。这种光寻址能力可用于可视化pH值或特定化学物质浓度的空间分布，在化学、材料科学、生物学和医学领域具有潜在应用。在本综述中，将这种化学成像传感器技术的特点与其他技术的特点进行了比较。描述了化学成像传感器系统的仪器设备、操作原理和各种测量模式。本综述讨论并总结了当前的先进技术，特别是在空间分辨率和测量速度方面；例如，利用LAPS已实现了亚微米范围内的高空间分辨率和每秒数万像素范围内的读出速度。还讨论了将该技术与微流控装置相结合的可能性以及其他潜在的未来发展方向。
