Miyamoto Ko-Ichiro, Sato Takuya, Abe Minami, Wagner Torsten, Schöning Michael J, Yoshinobu Tatsuo
Department of Electronic Engineering, Tohoku University, 6-6-05 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Institute of Nano- and Biotechnologies, Aachen University of Applied Sciences, Heinrich-Mußmann-Str. 1, Jülich 52428, Germany.
Micromachines (Basel). 2016 Jul 1;7(7):111. doi: 10.3390/mi7070111.
A plug-based microfluidic system based on the principle of the light-addressable potentiometric sensor (LAPS) is proposed. The LAPS is a semiconductor-based chemical sensor, which has a free addressability of the measurement point on the sensing surface. By combining a microfluidic device and LAPS, ion sensing can be performed anywhere inside the microfluidic channel. In this study, the sample solution to be measured was introduced into the channel in a form of a plug with a volume in the range of microliters. Taking advantage of the light-addressability, the position of the plug could be monitored and pneumatically controlled. With the developed system, the pH value of a plug with a volume down to 400 nL could be measured. As an example of plug-based operation, two plugs were merged in the channel, and the pH change was detected by differential measurement.
提出了一种基于光寻址电位传感器（LAPS）原理的基于塞子的微流控系统。LAPS是一种基于半导体的化学传感器，其在传感表面上的测量点具有自由寻址能力。通过将微流控装置与LAPS相结合，可以在微流控通道内的任何位置进行离子传感。在本研究中，待测量的样品溶液以微升范围内体积的塞子形式引入通道。利用光寻址能力，可以监测塞子的位置并进行气动控制。利用所开发的系统，可以测量体积低至400 nL的塞子的pH值。作为基于塞子操作的一个例子，两个塞子在通道中合并，并通过差分测量检测pH变化。
