中国西部牛病毒性腹泻病毒的分子检测与基因分型。
Molecular detection and genotyping of bovine viral diarrhea virus in Western China.
机构信息
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
出版信息
BMC Vet Res. 2021 Feb 2;17(1):66. doi: 10.1186/s12917-021-02747-7.
BACKGROUND
Bovine viral diarrhea virus (BVDV) is an important global viral pathogen of cattle and other ruminants. To survey the infection rate and genetic diversity of BVDV in western China, a total of 1234 serum samples from 17 herds of dairy cattle, beef cattle and yak in 4 provinces were collected in 2019.
RESULTS
All the 1234 serum samples were screened individually for BVDV by RT-PCR. Our results demonstrated that the average positive rate of BVDV was 7.2% (89/1234) in animals and 82.4% (14/17) in herds. Thirteen BVDV strains were isolated from RT-PCR positive clinical samples and they were all NCP biotype. BVDV-1a and 1c subgenotypes were identified from 22 selected virus isolates in 14 BVDV-positive herds. These results confirmed that BVDV-1a and BVDV-1c were circulating in western China, similar to the BVDV epidemics in cattle in other regions of China.
CONCLUSIONS
This study provides data for monitoring and vaccination strategies of BVDV in western China.
背景
牛病毒性腹泻病毒（BVDV）是一种重要的全球牛和其他反刍动物的病毒病原体。为了调查中国西部 BVDV 的感染率和遗传多样性，2019 年从中国 4 个省的 17 个奶牛、肉牛和牦牛群中采集了 1234 份血清样本。
结果
通过 RT-PCR 对 1234 份血清样本进行了单独的 BVDV 筛查。结果显示，动物中的 BVDV 平均阳性率为 7.2%（89/1234），群体中的阳性率为 82.4%（14/17）。从 RT-PCR 阳性的临床样本中分离出了 13 株 BVDV 株，均为 NCP 生物型。从 14 个 BVDV 阳性群的 22 个选定病毒分离株中鉴定出了 BVDV-1a 和 1c 亚群。这些结果证实了 BVDV-1a 和 BVDV-1c 在中国西部流行，与中国其他地区牛的 BVDV 流行情况相似。
结论
本研究为中国西部 BVDV 的监测和疫苗接种策略提供了数据。
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