Dong Biqin, Almassalha Luay M, Soetikno Brian T, Chandler John E, Nguyen The-Quyen, Urban Ben E, Sun Cheng, Zhang Hao F, Backman Vadim
Opt Express. 2017 Apr 3;25(7):7929-7944. doi: 10.1364/OE.25.007929.
We report detailed characterizations of stochastic fluorescence switching of unmodified nucleic acids under visible light illumination. Although the fluorescent emission from nucleic acids under the visible light illumination has long been overlooked due to their apparent low absorption cross section, our quantitative characterizations reveal the high quantum yield and high photon count in individual fluorescence emission events of nucleic acids at physiological concentrations. Owing to these characteristics, the stochastic fluorescence switching of nucleic acids could be comparable to that of some of the most potent exogenous fluorescence probes for localization-based super-resolution imaging. Therefore, utilizing the principle of single-molecule photon-localization microscopy, native nucleic acids could be ideal candidates for optical label-free super-resolution imaging.
我们报告了在可见光照射下未修饰核酸的随机荧光开关的详细表征。尽管由于核酸明显较低的吸收截面，其在可见光照射下的荧光发射长期以来一直被忽视，但我们的定量表征揭示了在生理浓度下核酸单个荧光发射事件中的高量子产率和高光子计数。由于这些特性，核酸的随机荧光开关可与一些用于基于定位的超分辨率成像的最有效的外源性荧光探针相媲美。因此，利用单分子光子定位显微镜原理，天然核酸可能是无光学标记超分辨率成像的理想候选物。
