Komoto Satoshi, Tacharoenmuang Ratana, Guntapong Ratigorn, Ide Tomihiko, Sinchai Phakapun, Upachai Sompong, Fukuda Saori, Yoshikawa Tetsushi, Tharmaphornpilas Piyanit, Sangkitporn Somchai, Taniguchi Koki
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
J Gen Virol. 2017 Apr;98(4):532-538. doi: 10.1099/jgv.0.000722. Epub 2017 Apr 6.
An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea. In this study, we sequenced and characterized the complete genome of strain KKL-117. On full-genomic analysis, strain KKL-117 was found to have the following genotype constellation: G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The non-G/P genotype constellation of this strain (I5-R1-C1-M1-A8-N1-T1-E1-H1) is commonly shared with rotavirus strains from pigs. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin. Our observations provide important insights into the dynamic interactions between human and porcine rotavirus strains.
在一名因严重腹泻住院的10个月大儿童的粪便标本中，鉴定出一种具有G9P[23]基因型的罕见轮状病毒株（RVA/Human-wt/THA/KKL-117/2014/G9P[23]）。在本研究中，我们对KKL-117株的全基因组进行了测序和特征分析。通过全基因组分析，发现KKL-117株具有以下基因型组合：G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1。该毒株的非G/P基因型组合（I5-R1-C1-M1-A8-N1-T1-E1-H1）通常与猪的轮状病毒株相同。此外，系统发育分析表明，KKL-117株的11个基因似乎均起源于猪。我们的观察结果为深入了解人源和猪源轮状病毒株之间的动态相互作用提供了重要见解。
