Sibley School of Mechanical and Aerospace Engineering, Cornell University , Ithaca, New York 14853, United States.
School of Applied and Engineering Physics, Cornell University , Ithaca, New York 14853, United States.
Anal Chem. 2017 May 2;89(9):5095-5100. doi: 10.1021/acs.analchem.7b00638. Epub 2017 Apr 13.
The quantification of analyte concentrations using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immunoassays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present at very high concentrations. In this paper, we present a reaction kinetics-based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy even in the hook effect range.
使用侧向流动分析定量分析物浓度是一种低成本、用户友好的替代传统基于实验室的分析方法。然而，夹心型免疫测定法通常受到高剂量钩状效应的限制，当分析物浓度非常高时，会导致结果假性偏低。在本文中，我们提出了一种基于反应动力学的技术，该技术解决了这个问题，显著增加了这些设备的动态范围。我们使用传统的夹心侧向流动免疫测定法、便携式成像设备和移动界面，通过在生理范围内大量量化人血清中的 C 反应蛋白浓度来演示该技术。该技术可应用于任何受钩状效应限制的夹心侧向流动分析，即使在钩状效应范围内也具有很高的准确性。
