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大肠杆菌K-12中铁超氧化物歧化酶基因（sodB）的克隆

Cloning of the iron superoxide dismutase gene (sodB) in Escherichia coli K-12.

作者信息

Sakamoto H, Touati D

出版信息

J Bacteriol. 1984 Jul;159(1):418-20. doi: 10.1128/jb.159.1.418-420.1984.

DOI:10.1128/jb.159.1.418-420.1984
PMID:6330047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215652/
Abstract

A clone overproducing iron superoxide dismutase has been isolated from an Escherichia coli cosmid bank. Subcloning located the gene responsible for iron superoxide dismutase overproduction on a 6.6-kilobase PstI restriction endonuclease fragment. Maxicell analysis, followed by immunological identification of iron superoxide dismutase protein, demonstrated that the structural gene, sodB, of iron superoxide dismutase has been cloned.

摘要

已从大肠杆菌黏粒文库中分离出一个过量产生铁超氧化物歧化酶的克隆。亚克隆将负责过量产生铁超氧化物歧化酶的基因定位在一个6.6千碱基的PstI限制性内切酶片段上。通过对铁超氧化物歧化酶蛋白进行免疫鉴定的最大细胞分析表明，铁超氧化物歧化酶的结构基因sodB已被克隆。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/174cd27c555e/jbacter00230-0427-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/314216f32641/jbacter00230-0426-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/992dde65b18b/jbacter00230-0427-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/174cd27c555e/jbacter00230-0427-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/314216f32641/jbacter00230-0426-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/992dde65b18b/jbacter00230-0427-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1b3/215652/174cd27c555e/jbacter00230-0427-b.jpg

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