Park M K, Myers R A, Marzella L
Department of Pathology, School of Medicine, University of Maryland, Baltimore 21201.
Antimicrob Agents Chemother. 1993 Jan;37(1):120-2. doi: 10.1128/AAC.37.1.120.
Hyperoxia prolongs the postantibiotic effect (PAE) of the aminoglycoside tobramycin in Pseudomonas aeruginosa. We tested the hypothesis that the PAE is prolonged because hyperoxia increases free radical flux while tobramycin inhibits the induction of antioxidant defenses. Exposure of P. aeruginosa to hyperoxia (100% O2) for 1 h increased superoxide dismutase, catalase, and glutathione levels. In the presence of tobramycin (1x the MIC), the induction of antioxidant defenses by hyperoxia was nearly abrogated. Neither preexposure of P. aeruginosa to hyperoxia nor supplementation with the antioxidants copper(II) (diisopropylsalicylate)2 (superoxide dismutase-like), catalase, or dimethyl sulfoxide abolished prolongation of the PAE of tobramycin induced by hyperoxia.
高氧可延长氨基糖苷类抗生素妥布霉素对铜绿假单胞菌的抗生素后效应（PAE）。我们检验了这样一个假说，即PAE延长是因为高氧增加了自由基通量，而妥布霉素抑制了抗氧化防御的诱导。将铜绿假单胞菌暴露于高氧（100% O2）1小时可增加超氧化物歧化酶、过氧化氢酶和谷胱甘肽水平。在妥布霉素（1倍MIC）存在的情况下，高氧对抗氧化防御的诱导几乎被消除。无论是将铜绿假单胞菌预先暴露于高氧，还是补充抗氧化剂铜（II）（二异丙基水杨酸）2（超氧化物歧化酶样）、过氧化氢酶或二甲基亚砜，都不能消除高氧诱导的妥布霉素PAE延长。
