Pilownic Katerine Jahnecke, Carvalho Ceci Nunes, Romano Ana Regina, Morgental Renata Dornelles, Shen Ya, Haapasalo Markus, Pappen Fernanda Geraldo
PhD student, Faculty of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Professor, Endodontics Division, Faculty of Dentistry, University Center of Maranhão, Uni-Ceuma, São Luís, Maranhão, Brazil.
Pediatr Dent. 2017 Mar 15;39(2):145-149.
The purpose of this study was to evaluate the effect of five endodontic materials for primary teeth on in vitro mixed-species biofilms.
Zinc oxide eugenol (ZOE) cement, Vitapex, Calen paste thickened with zinc oxide (ZO), pure calcium hydroxide (Ca(OH)2) paste, and iodoform were evaluated. Sterile water was used as a control. Mixed-species biofilm was incubated in anaerobic conditions for 21 days. The biofilm specimens were placed in contact with the endodontic materials for periods of seven and 30 days. The biofilm was studied by using confocal laser scanning microscopy. The cell viability ratio was calculated. The results were analyzed using analysis of variance and Tukey tests.
There was a statistically significant difference between groups at both seven and 30 days (F equals 73,073, P=0.00). After 30 days, 69 percent, 51 percent, and 35 percent of the biofilm volume fluoresced red, indicating the proportion of bacteria killed by iodoform, Vitapex, and ZOE cement, respectively. Calen plus ZO and pure Ca(OH)2 paste were the least effective materials against the biofilms.
Pure iodoform paste and iodoform containing Vitapex were the most effective materials against the biofilms. Vitapex appears to be a suitable endodontic material for primary teeth.
本研究旨在评估五种乳牙牙髓材料对体外混合菌种生物膜的影响。
对氧化锌丁香酚（ZOE）水门汀、Vitapex、用氧化锌增稠的卡伦糊剂（ZO）、纯氢氧化钙（Ca(OH)₂）糊剂和碘仿进行了评估。使用无菌水作为对照。混合菌种生物膜在厌氧条件下培养21天。将生物膜标本与牙髓材料接触7天和30天。使用共聚焦激光扫描显微镜研究生物膜。计算细胞活力比率。结果采用方差分析和Tukey检验进行分析。
在7天和30天时，各组之间均存在统计学上的显著差异（F = 73,073，P = 0.00）。30天后，生物膜体积的69%、51%和35%发出红色荧光，分别表明被碘仿、Vitapex和ZOE水门汀杀死的细菌比例。卡伦加ZO和纯Ca(OH)₂糊剂是对抗生物膜效果最差的材料。
纯碘仿糊剂和含碘仿的Vitapex是对抗生物膜最有效的材料。Vitapex似乎是一种适用于乳牙的牙髓材料。
