具有不同抗生素敏感性的三株[具体物种名称未给出]的种内比较基因组学
Intraspecies comparative genomics of three strains of with different antibiotic sensitivity.
作者信息
Liao Hsiao-Mei, Chao Chien-Chung, Lei Haiyan, Li Bingjie, Tsai Shien, Hung Guo-Chiuan, Ching Wei-Mei, Lo Shyh-Ching
机构信息
Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.
Naval Medical Research Center, Silver Spring, MD, USA; Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
出版信息
Genom Data. 2017 Mar 28;12:84-88. doi: 10.1016/j.gdata.2017.03.012. eCollection 2017 Jun.
We recently reported the genome of (OT) strain Karp (GenBank Accession #: NZ_LYMA00000000.2, https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2) with > 2 Mb in size through clone-based sequencing and high throughput genomic shotgun sequencing (HTS). The genomes of OT strains AFSC4 and AFSC7 were similarly sequenced by HTS Since strains AFSC4 (GenBank Accession #: NZ_LYMT00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035784408) and AFSC7 (GenBank Accession #: NZ_LYMB00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035854767) were more resistant to antibiotics than strain Karp, we conducted comparative analysis of the three draft genomes annotated by RAST server aimed to identify possible genetic bases of difference in microbial antibiotic sensitivity. Intraspecies comparative genomics analysis of the three OT strains revealed that two ORFs encoding hypothetical proteins in both strains AFSC4 and AFSC7 are absent in strain Karp.
我们最近通过基于克隆的测序和高通量基因组鸟枪法测序（HTS）报道了（OT）菌株Karp的基因组（GenBank登录号：NZ_LYMA00000000.2，https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2），其大小超过2 Mb。OT菌株AFSC4和AFSC7的基因组也通过HTS进行了类似的测序。由于菌株AFSC4（GenBank登录号：NZ_LYMT00000000.1，https://www.ncbi.nlm.nih.gov/nuccore/1035784408）和AFSC7（GenBank登录号：NZ_LYMB00000000.1，https://www.ncbi.nlm.nih.gov/nuccore/1035854767）比菌株Karp对抗生素更具抗性，我们对由RAST服务器注释的三个草图基因组进行了比较分析，旨在确定微生物抗生素敏感性差异的可能遗传基础。对这三个OT菌株的种内比较基因组学分析表明，菌株Karp中不存在AFSC4和AFSC7菌株中两个编码假设蛋白质的开放阅读框。
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