Dong Li-Mei, Zhang Mei, Xu Qiao-Lin, Zhang Qiang, Luo Bi, Luo Qing-Wen, Liu Wen-Bin, Tan Jian-Wen
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Molecules. 2017 Apr 8;22(4):592. doi: 10.3390/molecules22040592.
Two new thymol derivatives, 7,9-diisobutyryloxy-8-ethoxythymol () and 7-acetoxy-8-methoxy-9-isobutyryloxythymol (), were isolated from fresh roots of , together with four known compounds, 7,9-di-isobutyryloxy-8-methoxythymol (), 9-oxoageraphorone (), (-)-isochaminic acid () and (1α,6α)-10-hydroxycar-3-ene-2-one (). Their structures were established on the basis of detailed spectroscopic analysis, and they were all isolated from the roots of for the first time. All the compounds were tested for their in vitro antibacterial activity toward three Gram-positive and two Gram-negative bacterial strains. Thymol derivatives - only selectively showed slight in vitro bacteriostatic activity toward three Gram-positive bacteria. The two known carene-type monoterpenes and were found to show moderate in vitro antibacterial activity against all five tested bacterial strains, with MIC values from 15.6 to 62.5 μg/mL. In addition, compounds and were further revealed to show in vitro cytotoxicity against human tumor A549, HeLa and HepG2 cell lines, with IC values ranging from 18.36 to 41.87 μM. However, their cytotoxic activities were inferior to those of reference compound adriamycin.
从新鲜的[植物名称]根部分离出两种新的百里香酚衍生物，7,9 - 二异丁酰氧基 - 8 - 乙氧基百里香酚（）和7 - 乙酰氧基 - 8 - 甲氧基 - 9 - 异丁酰氧基百里香酚（），以及四种已知化合物，7,9 - 二异丁酰氧基 - 8 - 甲氧基百里香酚（）、9 - 氧代紫穗槐二烯（）、（ - ） - 异卡米宁酸（）和（1α,6α） - 10 - 羟基蒈 - 3 - 烯 - 2 - 酮（）。它们的结构通过详细的光谱分析得以确定，并且均首次从[植物名称]根部分离得到。对所有化合物针对三种革兰氏阳性和两种革兰氏阴性细菌菌株进行了体外抗菌活性测试。百里香酚衍生物 - 仅对三种革兰氏阳性细菌选择性地表现出轻微的体外抑菌活性。发现两种已知的蒈烯型单萜和对所有五种测试细菌菌株均表现出中等的体外抗菌活性，最低抑菌浓度（MIC）值为15.6至62.5μg/mL。此外，化合物和进一步显示出对人肿瘤A549、HeLa和HepG2细胞系的体外细胞毒性，IC值范围为18.36至41.87μM。然而，它们的细胞毒性活性低于参考化合物阿霉素。
