Sakai T, Araki T, Ushio K
Center of Occupational Medicine, Tokyo Labor Accident Hospital, Japan.
Br J Ind Med. 1988 Jun;45(6):420-5. doi: 10.1136/oem.45.6.420.
A simple method for determining pyrimidine 5'-nucleotidase (P5N) activity in whole blood has been developed, inhibiting the plasma activity for UMP-hydrolysis by concanavalin (Con A). Con A specifically inhibits the activity of plasma 5'-nucleotidase (5N) but does not affect erythrocyte P5N activity. The anticoagulant EDTA partially inhibits 5N activity but slightly activates P5N. P5N activity determined by the present method with heparinised blood and Con A was comparable with that by the method reported previously and correlated well with blood lead concentrations. The mean value and SD for P5N activity in normal subjects (n = 72) not exposed to lead are 16.2 and 2.5 mumole/h/g Hb, respectively. The present method can eliminate not only the isolation step of RBC but also Hb determination, the activity being expressed as mumole/h/l blood or RBC. Thus the procedures are so simplified that the assay may be used as a routine test for mass screening of lead exposure.
已开发出一种测定全血中嘧啶5'-核苷酸酶（P5N）活性的简单方法，通过伴刀豆球蛋白（Con A）抑制血浆中UMP水解活性。Con A特异性抑制血浆5'-核苷酸酶（5N）的活性，但不影响红细胞P5N活性。抗凝剂EDTA部分抑制5N活性，但轻微激活P5N。用本法测定肝素化血液和Con A时的P5N活性与先前报道的方法相当，且与血铅浓度密切相关。未接触铅的正常受试者（n = 72）的P5N活性平均值和标准差分别为16.2和2.5微摩尔/小时/克血红蛋白。本法不仅可以省去红细胞分离步骤，还可以省去血红蛋白测定步骤，活性以微摩尔/小时/升血液或红细胞表示。因此，该程序大大简化，该测定法可作为铅暴露大规模筛查的常规检测方法。
