红细胞精氨酸酶、嘧啶5'-核苷酸酶（P5N）和脱氧嘧啶5'-核苷酸酶（dP5N）作为铅暴露指标。
Erythrocyte arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N) as indices of lead exposure.
作者信息
Cook L R, Angle C R, Stohs S J
出版信息
Br J Ind Med. 1986 Jun;43(6):387-90. doi: 10.1136/oem.43.6.387.
The activities of three erythrocyte (rbc) enzymes, arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N), were compared in 16 lead workers and 14 age matched controls as correlates of blood lead (PbB) and unextracted zinc protoporphyrin (EP) concentrations. Subjects with PbB of 0.9-2.5 microM (19-52 micrograms/dl) had 6.5 +/- 0.6 IU of P5N activity with uridine monophosphate (UMP) as substrate, significantly less (p less than 0.001) than the 12.0 +/- 0.7 IU activity of controls with PbB 0.3-0.6 microM (6-12 micrograms/dl). The mean activity of rbc dP5N with either deoxyuridine monophosphate (dUMP) or thymidine monophosphate as substrate, and of rbc arginase, did not differentiate the two groups. The correlation coefficients of ln PbB with the selected substrates for P5N and dP5N were: UMP, r = -0.75; dUMP, r = -0.61; TMP, r = -0.23. The correlation coefficient of ln PbB and arginase activity was -0.03. Rbc P5N (UMPase) is a significant correlate of PbB, equivalent to rbc protoporphyrin. HPLC assay of rbc UMPase activity is a sensitive and rapid assay that appears to meet criteria for a reliable clinical laboratory index of blood lead concentrations.
比较了16名铅作业工人和14名年龄匹配的对照者的三种红细胞（rbc）酶——精氨酸酶、嘧啶5'-核苷酸酶（P5N）和脱氧嘧啶5'-核苷酸酶（dP5N）的活性，作为血铅（PbB）和未提取的锌原卟啉（EP）浓度的相关指标。血铅浓度为0.9 - 2.5微摩尔/升（19 - 52微克/分升）的受试者，以单磷酸尿苷（UMP）为底物时，P5N活性为6.5±0.6国际单位，显著低于血铅浓度为0.3 - 0.6微摩尔/升（6 - 12微克/分升）的对照者的12.0±0.7国际单位（p＜0.001）。以单磷酸脱氧尿苷（dUMP）或单磷酸胸腺嘧啶核苷为底物时，红细胞dP5N的平均活性以及红细胞精氨酸酶的活性，均未区分这两组。ln PbB与P5N和dP5N所选底物的相关系数分别为：UMP，r = -0.75；dUMP，r = -0.61；TMP，r = -0.23。ln PbB与精氨酸酶活性的相关系数为 -0.03。红细胞P5N（UMP酶）是血铅的一个重要相关指标，等同于红细胞原卟啉。红细胞UMP酶活性的高效液相色谱测定法是一种灵敏且快速的检测方法，似乎符合作为血铅浓度可靠临床实验室指标的标准。
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