Ozdal Murat, Ozdal Ozlem Gur, Sezen Alev, Algur Omer Faruk, Kurbanoglu Esabi Basaran
Department of Biology, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey.
3 Biotech. 2017 May;7(1):23. doi: 10.1007/s13205-017-0605-0. Epub 2017 Apr 11.
Indole acetic acid (IAA) is a plant growth-promoting hormone used in agriculture; therefore, its continuous production is of paramount importance. IAA-producing eight bacteria were isolated from the rhizosphere of Verbascum vulcanicum. Among them, Arthrobacter agilis A17 gave maximum IAA production (75 mg/L) and this strain was used to immobilization studies. The A. agilis A17 cells were immobilized in calcium alginate for the production of IAA. Optimization of process parameters for IAA production was carried out to enhance IAA production using immobilized cells. The maximal production of IAA was 520 mg/L under the following optimal conditions: 1% mannitol, 30 °C, pH 8.0, and 24 h incubation. It was determined that the immobilized cells could be reused (13 times) for the production of IAA.
吲哚乙酸（IAA）是一种用于农业的促进植物生长的激素；因此，其持续生产至关重要。从毛泡桐根际分离出八株产IAA的细菌。其中，敏捷节杆菌A17的IAA产量最高（75毫克/升），该菌株用于固定化研究。将敏捷节杆菌A17细胞固定在海藻酸钙中以生产IAA。为提高固定化细胞生产IAA的产量，对IAA生产的工艺参数进行了优化。在以下最佳条件下，IAA的最大产量为520毫克/升：1%甘露醇、30℃、pH 8.0和24小时培养。结果表明，固定化细胞可重复用于生产IAA（13次）。
