Central Research Facility, Rubber Technology Centre, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India.
Department of Neurological Sciences, Rush University Medical Centre, Chicago, IL, USA.
Sci Rep. 2017 Apr 12;7:46412. doi: 10.1038/srep46412.
Lignin, one of the most abundant renewable feedstock, is used to develop a biocompatible hydrogel as anti-infective ointment. A hydrophilic polyoxazoline chain is grafted through ring opening polymerization, possess homogeneous spherical nanoparticles of 10-15 nm. The copolymer was covalently modified with triazole moiety to fortify the antimicrobial and antibiofilm activities. The hydrogel was capable of down regulating the expression level of IL-1β in LPS induced macrophage cells, and to cause significant reduction of iNOS production. It supported cellular anti-inflammatory activity which was confirmed with luciferase assay, western blot, and NF-κB analysis. This novel lignin-based hydrogel tested in-vivo has shown the abilities to prevent infection of burn wound, aid healing, and an anti-inflammatory dressing material. The hydrogel reported here provides a new material platform to introduce a cost-effective and efficient ointment option after undertaking further work to look at its use in the area of clinical practice.
木质素是最丰富的可再生原料之一，被用来开发一种具有生物相容性的水凝胶作为抗感染软膏。通过开环聚合接枝了亲水性聚恶唑啉链，得到了 10-15nm 的均匀球形纳米粒子。共聚物通过三唑部分进行了共价修饰，以增强其抗菌和抗生物膜活性。水凝胶能够下调 LPS 诱导的巨噬细胞中 IL-1β 的表达水平，并显著减少 iNOS 的产生。它支持细胞抗炎活性，这一点通过荧光素酶测定、western blot 和 NF-κB 分析得到了证实。这种新型基于木质素的水凝胶在体内测试中表现出了预防烧伤创面感染、促进愈合和作为抗炎敷料的能力。本文报道的水凝胶为进一步研究其在临床实践中的应用提供了一种具有成本效益和高效的软膏选择的新材料平台。
