Nyman Tuula A, Lorey Martina B, Cypryk Wojciech, Matikainen Sampsa
a Department of Immunology , Institute of Clinical Medicine, University of Oslo and Rikshospitalet Oslo , Oslo , Norway.
b Rheumatology , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
Expert Rev Proteomics. 2017 May;14(5):395-407. doi: 10.1080/14789450.2017.1319768. Epub 2017 Apr 24.
The immune system is our defense system against microbial infections and tissue injury, and understanding how it works in detail is essential for developing drugs for different diseases. Mass spectrometry-based proteomics can provide in-depth information on the molecular mechanisms involved in immune responses. Areas covered: Summarized are the key immunology findings obtained with MS-based proteomics in the past five years, with a focus on inflammasome activation, global protein secretion, mucosal immunology, immunopeptidome and T cells. Special focus is on extracellular vesicle-mediated protein secretion and its role in immune responses. Expert commentary: Proteomics is an essential part of modern omics-scale immunology research. To date, MS-based proteomics has been used in immunology to study protein expression levels, their subcellular localization, secretion, post-translational modifications, and interactions in immune cells upon activation by different stimuli. These studies have made major contributions to understanding the molecular mechanisms involved in innate and adaptive immune responses. New developments in proteomics offer constantly novel possibilities for exploring the immune system. Examples of these techniques include mass cytometry and different MS-based imaging approaches which can be widely used in immunology.
免疫系统是我们抵御微生物感染和组织损伤的防御系统，详细了解其工作原理对于开发针对不同疾病的药物至关重要。基于质谱的蛋白质组学能够提供有关免疫反应所涉及分子机制的深入信息。涵盖领域：总结了过去五年基于质谱的蛋白质组学所获得的关键免疫学发现，重点关注炎性小体激活、整体蛋白质分泌、黏膜免疫学、免疫肽组和T细胞。特别关注细胞外囊泡介导的蛋白质分泌及其在免疫反应中的作用。专家评论：蛋白质组学是现代组学规模免疫学研究的重要组成部分。迄今为止，基于质谱的蛋白质组学已被用于免疫学研究蛋白质表达水平、它们的亚细胞定位、分泌、翻译后修饰以及免疫细胞在不同刺激激活后的相互作用。这些研究为理解先天和适应性免疫反应所涉及的分子机制做出了重大贡献。蛋白质组学的新发展为探索免疫系统不断提供新的可能性。这些技术的例子包括质谱流式细胞术和不同的基于质谱的成像方法，它们可广泛应用于免疫学。
