Pyle L E, Taylor T, Finch L R
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.
J Bacteriol. 1990 Dec;172(12):7265-8. doi: 10.1128/jb.172.12.7265-7268.1990.
Genomic restriction maps for the small colony (SC) strains (PG1, KH3J, Gladysdale, and V5) of Mycoplasma mycoides subsp. mycoides (the agent of contagious bovine pleuropneumonia) and for Mycoplasma strain PG50 (classified as bovine serogroup 7), with respective sizes of 1,280, 1,280, 1,260, 1,230, and 1,040 kbp, were compared with the map (1,200 kbp) for a large colony strain (Y goat) of M. mycoides subsp. mycoides. The number and order of all mapped restriction sites were fully conserved in the SC genomes, as were the approximate positions of mapped loci. A number of these restriction sites in the Y genome and some, but fewer, in the PG50 genome appeared to be conserved. The SC and large colony strains shared conservation in the relative positions of the mapped loci, except for rpoC.
丝状支原体山羊亚种的小菌落(SC)菌株(PG1、KH3J、Gladysdale和V5)以及PG50支原体菌株(分类为牛血清群7)的基因组限制性图谱,其大小分别为1280、1280、1260、1230和1040千碱基对,与丝状支原体山羊亚种的大菌落菌株(Y山羊)的图谱(1200千碱基对)进行了比较。所有定位的限制性酶切位点的数量和顺序在SC基因组中完全保守,定位基因座的大致位置也是如此。Y基因组中的许多这些限制性酶切位点以及PG50基因组中的一些(但较少)限制性酶切位点似乎是保守的。除了rpoC外,SC菌株和大菌落菌株在定位基因座的相对位置上具有保守性。