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电针刺激SD大鼠足三里穴对免疫细胞因子及脾脏CD4+T细胞的增强作用

Enhancement of immune cytokines and splenic CD4+ T cells by electroacupuncture at ST36 acupoint of SD rats.

作者信息

Chen Longyun, Xu Anli, Yin Nina, Zhao Min, Wang Zhigang, Chen Tao, Gao Yisheng, Chen Zebin

机构信息

Department of Biochemistry, Basic Medical College, Hubei University of Chinese Medicine, Wuhan, China.

Department of Anatomy, Basic Medical College, Hubei University of Chinese Medicine, Wuhan, China.

出版信息

PLoS One. 2017 Apr 13;12(4):e0175568. doi: 10.1371/journal.pone.0175568. eCollection 2017.

DOI:10.1371/journal.pone.0175568
PMID:28406959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5391063/
Abstract

Electroacupuncture at the ST36 acupoint can enhance the body's immune function. However, the mechanism for this enhancement has not been fully described. Our study was designed to investigate the effect of electroacupuncture on the immune function of Sprague-Dawley (SD) rats. The rats were randomly divided into three groups: a control group, a non-acupoint group (abdominal muscle acupuntured) and a ST36 acupoint group. Our results showed that successive electroacupuncture at the ST36 acupoint for 3 d significantly enhanced the interferon-γ (IFN-γ) level in the serum of SD rats. The results also showed that the serum and extracts from spleen cells of the ST36 acupoint group contained higher levels of interleukin (IL)-2 and IL-17 compared to those of the other two groups. Immunohistochemical analysis showed that electroacupuncture applied to the ST36 acupoint enhanced the expression level of CD4 in spleen cells. Furthermore, it was observed that CD4 co-localized with transient receptor potential vanilloid (TRPV) channels at the membrane of splenic CD4+ T cells and the expression level of CD4 was related to TRPV channels in the electroacupuncture treatment. These observations indicated that electroacupuncture stimulation at the ST36 acupoint enhanced the level of immune cytokines and splenic CD4+ T cells through TRPV channels in this system.

摘要

电针刺激足三里穴位可增强机体免疫功能。然而，这种增强作用的机制尚未完全阐明。本研究旨在探讨电针刺激对Sprague-Dawley（SD）大鼠免疫功能的影响。将大鼠随机分为三组：对照组、非穴位组（针刺腹部肌肉）和足三里穴位组。我们的研究结果表明，连续3天电针刺激足三里穴位可显著提高SD大鼠血清中干扰素-γ（IFN-γ）水平。结果还显示，与其他两组相比，足三里穴位组大鼠血清及脾细胞提取物中白细胞介素（IL）-2和IL-17水平更高。免疫组织化学分析表明，电针刺激足三里穴位可提高脾细胞中CD4的表达水平。此外，观察发现CD4与脾CD4+T细胞膜上的瞬时受体电位香草酸亚型（TRPV）通道共定位，且在电针治疗中CD4的表达水平与TRPV通道有关。这些观察结果表明，在该系统中，电针刺激足三里穴位可通过TRPV通道提高免疫细胞因子水平及脾CD4+T细胞数量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/720f748693bf/pone.0175568.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/3a7856f8ecbf/pone.0175568.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/63630854b1f5/pone.0175568.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/e5504b54544c/pone.0175568.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/73577e85599a/pone.0175568.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/7b795578fefe/pone.0175568.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/e8d5fcbcf2ee/pone.0175568.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/720f748693bf/pone.0175568.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/3a7856f8ecbf/pone.0175568.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/63630854b1f5/pone.0175568.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/e5504b54544c/pone.0175568.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/73577e85599a/pone.0175568.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/7b795578fefe/pone.0175568.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/e8d5fcbcf2ee/pone.0175568.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/656c/5391063/720f748693bf/pone.0175568.g007.jpg

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