Yue Dong-Mei, Ma Jian-Gang, Li Fa-Cai, Hou Jun-Ling, Zheng Wen-Bin, Zhao Quan, Zhang Xiao-Xuan, Zhu Xing-Quan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesLanzhou, China.
College of Animal Science and Technology, Jilin Agricultural UniversityChangchun, China.
Front Microbiol. 2017 Mar 31;8:565. doi: 10.3389/fmicb.2017.00565. eCollection 2017.
is an important zoonotic parasite. It can infect virtually all animal species and has a global distribution. However, the prevalence of in donkeys () has only been reported in Algeria and Spain, and no information is available concerning genotypes of in donkeys worldwide. In the present study, a total of 301 donkey fecal samples (48 from Jilin Province, 224 from Shandong Province and 29 from Liaoning Province) were collected and examined by PCR amplification of the internal transcribed spacer (ITS) region. The overall . prevalence was 5.3% (16/301), with 6.3% (3/48) in Jilin Province, 4.9% (11/224) in Shandong Province, and 6.9% (2/29) in Liaoning Province. Prevalence in different age groups ranged from 4.2 to 5.5%. . prevalence in donkeys sampled in different seasons varied from 4.2 to 6.5%. Altogether, four genotypes were identified in this study, with two known genotypes (J and D) and two novel genotypes (NCD-1and NCD-2). Phylogenetic analysis revealed that genotypes D, NCD-1 and NCD-2 belonged to group 1, while the remaining genotype J was clustered into group 2. These findings revealed the occurrence of in donkeys in China for the first time. Moreover, the present study also firstly genotyped the . in donkeys worldwide. These findings extend the distribution of genotypes and provide baseline data for controlling infection in donkeys, other animals and humans.
是一种重要的人畜共患寄生虫。它几乎可以感染所有动物物种，并且在全球范围内都有分布。然而，仅在阿尔及利亚和西班牙报道过驴感染的情况，关于全球范围内驴感染的基因型尚无相关信息。在本研究中，共收集了301份驴粪便样本（48份来自吉林省，224份来自山东省，29份来自辽宁省），并通过对内部转录间隔区（ITS）区域进行PCR扩增来检测。总体感染率为5.3%（16/301），其中吉林省为6.3%（3/48），山东省为4.9%（11/224），辽宁省为6.9%（2/29）。不同年龄组的感染率在4.2%至5.5%之间。不同季节采集的驴样本的感染率在4.2%至6.5%之间变化。本研究共鉴定出四种基因型，其中两种为已知基因型（J和D），两种为新基因型（NCD - 1和NCD - 2）。系统发育分析表明，基因型D、NCD - 1和NCD - 2属于第1组，而其余的基因型J则聚类到第2组。这些发现首次揭示了在中国驴中存在该寄生虫感染。此外，本研究还首次对全球范围内驴感染的该寄生虫进行了基因分型。这些发现扩展了该寄生虫基因型的分布范围，并为控制驴、其他动物和人类的该寄生虫感染提供了基线数据。
