Anaya-Reza Omar, Lopez-Arenas Teresa
Posgrado en Energía y Medio Ambiente, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico.
Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico City, Mexico.
Bioprocess Biosyst Eng. 2017 Jul;40(7):1033-1048. doi: 10.1007/s00449-017-1766-2. Epub 2017 Apr 13.
L-Lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. In this work, the production process of L-lysine-HCl is studied using a systematic approach based on modeling and simulation, which supports decision making in the early stage of process design. The study considers two analysis stages: first, the dynamic analysis of the fermentation reactor, where the conversion of sugars from sugarcane molasses to L-lysine with a strain of Corynebacterium glutamicum is carried out. In this stage, the operation mode (either batch or fed batch) and operating conditions of the fermentation reactor are defined to reach the maximum technical criteria. Afterwards, the second analysis stage relates to the industrial production process of L-lysine-HCl, where the fermentation reactor, upstream processing, and downstream processing are included. In this stage, the influence of key parameters on the overall process performance is scrutinized through the evaluation of several technical, economic, and environmental criteria, to determine a profitable and sustainable design of the L-lysine production process. The main results show how the operating conditions, process design, and selection of evaluation criteria can influence in the conceptual design. The best plant design shows maximum product yield (0.31 g L-lysine/g glucose) and productivity (1.99 g/L/h), achieving 26.5% return on investment (ROI) with a payback period (PBP) of 3.8 years, decreasing water and energy consumption, and with a low potential environmental impact (PEI) index.
L-赖氨酸是一种必需氨基酸，它既可以通过化石原料的化学工艺生产，也可以通过微生物发酵生产，后者是一种更高效且环保的方法。在这项工作中，采用基于建模和模拟的系统方法对L-盐酸赖氨酸的生产过程进行了研究，该方法有助于在工艺设计的早期阶段进行决策。该研究考虑了两个分析阶段：首先，对发酵反应器进行动态分析，在此阶段，使用谷氨酸棒杆菌菌株将甘蔗废蜜中的糖转化为L-赖氨酸。在这个阶段，确定发酵反应器的操作模式（分批或补料分批）和操作条件，以达到最大技术标准。之后，第二个分析阶段涉及L-盐酸赖氨酸的工业生产过程，其中包括发酵反应器、上游加工和下游加工。在这个阶段，通过评估几个技术、经济和环境标准，仔细审查关键参数对整个工艺性能的影响，以确定L-赖氨酸生产工艺的盈利且可持续的设计。主要结果表明操作条件、工艺设计和评估标准的选择如何在概念设计中产生影响。最佳工厂设计显示出最大产品产量（0.31 g L-赖氨酸/ g葡萄糖）和生产率（1.99 g/L/h），投资回报率（ROI）达到26.5%，投资回收期（PBP）为3.8年，同时降低了水和能源消耗，并且具有较低的潜在环境影响（PEI）指数。
