Kupiec M, Petes T D
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
Genetics. 1988 Jul;119(3):549-59. doi: 10.1093/genetics/119.3.549.
Allelic and nonallelic (ectopic) recombination events were analyzed in a set of isogenic strains that carry marked Ty elements. We found that allelic recombination between Ty elements occurred at normal frequencies both in meiosis and mitosis. The marked Ty elements were involved in a large variety of different types of ectopic recombination and this variety was greater in mitosis than in meiosis. Allelic and ectopic recombination events occurred at similar frequencies in mitosis, but allelic recombination predominated in meiosis. Some of the types of ectopic mitotic recombination indicated the common occurrence of concerted recombination events. The length of homology represented by a delta element (330 bp) seemed to be sufficient for some types of mitotic and meiotic recombination.
在一组携带标记 Ty 元件的同基因菌株中分析了等位基因和非等位基因(异位)重组事件。我们发现 Ty 元件之间的等位基因重组在减数分裂和有丝分裂中均以正常频率发生。标记的 Ty 元件参与了多种不同类型的异位重组,且这种多样性在有丝分裂中比在减数分裂中更大。等位基因和异位重组事件在有丝分裂中以相似频率发生,但等位基因重组在减数分裂中占主导。一些类型的异位有丝分裂重组表明协同重组事件普遍存在。由 δ 元件(330 bp)代表的同源长度似乎足以支持某些类型的有丝分裂和减数分裂重组。