Ohara Masayuki, Takinoue Masahiro, Kawano Ryuji
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology (TUAT) , 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.
Department of Computer Science, Tokyo Institute of Technology , 4259 Nagatsuta-cho, Yokohama, Kanagawa 226-8502, Japan.
ACS Synth Biol. 2017 Jul 21;6(7):1427-1432. doi: 10.1021/acssynbio.7b00101. Epub 2017 Apr 25.
This paper describes an AND logic operation with amplification and transcription from DNA to RNA, using T7 RNA polymerase. All four operations, (0 0) to (1 1), with an enzyme reaction can be performed simultaneously, using four-droplet devices that are directly connected to a patch-clamp amplifier. The output RNA molecule is detected using a biological nanopore with single-molecule translocation. Channel current recordings can be obtained using the enzyme solution. The integration of DNA logic gates into electrochemical devices is necessary to obtain output information in a human-recognizable form. Our method will be useful for rapid and confined DNA computing applications, including the development of programmable diagnostic devices.
本文描述了一种利用T7 RNA聚合酶进行从DNA到RNA的扩增和转录的“与”逻辑运算。使用直接连接到膜片钳放大器的四滴液装置，可以同时进行所有四种操作，即从（0 0）到（1 1）的酶反应。使用具有单分子转运功能的生物纳米孔检测输出的RNA分子。可以使用酶溶液获得通道电流记录。将DNA逻辑门集成到电化学装置中对于以人类可识别的形式获得输出信息是必要的。我们的方法将有助于快速且受限的DNA计算应用，包括可编程诊断设备的开发。
