Keskin Zalike, Sendemir Urkmez Aylin, Hames E Esin
Department of Biomedical Engineering, Faculty of Engineering, Izmir University of Economics, Izmir, Turkey.
Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey; Department of Biomedical Technologies, Ege University, Izmir, Turkey; Central Research Test and Analysis Laboratories Research and Application Center (EGEMATAL), Ege University, Izmir, Turkey.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:1144-1153. doi: 10.1016/j.msec.2017.03.035. Epub 2017 Mar 6.
As it is known that bacterial cellulose (BC) is a biocompatible and natural biopolymer due to which it has a large set of biomedical applications. But still it lacks some desired properties, which limits its uses in many other applications. Therefore, the properties of BC need to be boosted up to an acceptable level. Here in this study for the first time, a new natural nanocomposite was produced by the incorporating keratin (isolated from human hair) to the BC (produced by Acetobacter xylinum) to enhance dermal fibroblast cells' attachment. Two different approaches were used in BC based nanocomposite production: in situ and post modifications. BC/keratin nanocomposites were characterized using SEM, FTIR, EDX, XRD, DSC and XPS analyses. Both production methods have yielded successful results for production of BC based nanocomposite-containing keratin. In vitro cell culture experiments performed with human skin keratinocytes and human skin fibroblast cells indicate the potential of the novel BC/keratin nanocomposites for use in skin tissue engineering.
众所周知，细菌纤维素（BC）是一种生物相容性天然生物聚合物，因此具有大量的生物医学应用。但它仍然缺乏一些理想的性能，这限制了它在许多其他应用中的使用。因此，需要将BC的性能提高到可接受的水平。在本研究中，首次通过将角蛋白（从人发中分离）掺入到由木醋杆菌产生的BC中，制备了一种新型天然纳米复合材料，以增强真皮成纤维细胞的附着。在基于BC的纳米复合材料生产中使用了两种不同的方法：原位修饰和后修饰。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、能量色散X射线光谱（EDX）、X射线衍射（XRD）、差示扫描量热法（DSC）和X射线光电子能谱（XPS）分析对BC/角蛋白纳米复合材料进行了表征。两种生产方法在含角蛋白的基于BC的纳米复合材料生产方面均取得了成功结果。用人皮肤角质形成细胞和人皮肤成纤维细胞进行的体外细胞培养实验表明了新型BC/角蛋白纳米复合材料在皮肤组织工程中的应用潜力。
