Tian Zizhu, Huang Lixun, Pei Xibo, Chen Junyu, Wang Tong, Yang Tao, Qin Han, Sui Lei, Wang Jian
West China School of Stomatology, Sichuan University, NO. 14, 3rd Section of Ren Min Nan Rd., Chengdu, Sichuan 610041, China.
School of Stomatology, Tianjin Medical University, NO. 12, Qixiangtai Rd. Heping District, Tianjin 300070, China.
Colloids Surf B Biointerfaces. 2017 Jul 1;155:150-158. doi: 10.1016/j.colsurfb.2017.04.012. Epub 2017 Apr 12.
In this study, three-dimensional reduced graphene oxide (3D-rGO) porous films were fabricated using a two-step electrochemical method, including an electrochemical deposition process for the self-assembly of GO and an electrochemical bubbling-based transfer. The morphology, physical properties, and phase composition of the 3D-rGO films were characterized, and the cellular bioactivities were evaluated using pre-osteoblasts (MC3T3-E1 cells). The attachment, proliferation and differentiation of the MC3T3-E1 cells on the 3D-rGO films was analyzed by scanning electron microscopy (SEM), Cell Counting Kit-8 (CCK-8) assays and live/dead cell staining, and alkaline phosphatase (ALP) activity assays, respectively. The expression of osteogenic-related genes in MC3T3-E1 cells was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the 3D-rGO films supported cell viability and proliferation, as well as significantly enhanced ALP activity and osteogenic-related genes (ALP, OPN, Runx2) expressions. Our findings indicate the promising potential of the 3D-rGO porous films for bone tissue engineering.
在本研究中，采用两步电化学方法制备了三维还原氧化石墨烯（3D-rGO）多孔膜，该方法包括用于氧化石墨烯自组装的电化学沉积过程和基于电化学鼓泡的转移过程。对3D-rGO膜的形态、物理性质和相组成进行了表征，并使用前成骨细胞（MC3T3-E1细胞）评估了细胞生物活性。分别通过扫描电子显微镜（SEM）、细胞计数试剂盒-8（CCK-8）测定、活/死细胞染色以及碱性磷酸酶（ALP）活性测定，分析了MC3T3-E1细胞在3D-rGO膜上的附着、增殖和分化情况。通过逆转录-聚合酶链反应（RT-PCR）评估了MC3T3-E1细胞中成骨相关基因的表达。结果表明，3D-rGO膜支持细胞活力和增殖，并显著增强了ALP活性和成骨相关基因（ALP、OPN、Runx2）的表达。我们的研究结果表明，3D-rGO多孔膜在骨组织工程方面具有广阔的应用前景。
