Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, 44519, Egypt.
Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, 44519, Egypt.
Biomed Pharmacother. 2017 Jun;90:731-739. doi: 10.1016/j.biopha.2017.04.026. Epub 2017 Apr 15.
The toxicological studies on silver nanoparticles (Ag-NPs) have become a hot topic over the past few decades due to their unique properties on the nanoscale and widespread in many commercial products that launched into the market recently. This study was undertaken to shed light on Ag-NPs toxicity on neurotransmitters with special emphasis on the impact of concurrent administration of rutin with Ag-NPs in the experimental rats. The oral administration of Ag-NPs in rats induced brain oxidative stress, significant alterations in neurotransmitters and amino acids. Furthermore, transcriptional levels of glutamatergic N-methyl-d-aspartate (NMDA) receptors, monoamino oxidases (MAO-A, MAO-B) and metallothionein-III (MT-III) showed a significant elevation in Ag-NPs intoxicated rats. Moreover, histological examinations revealed astrogliosis and demyelination of neurons concomitant with neuronal degeneration and vacuolation. Strikingly, oral administration of rutin counterbalanced the toxic effects triggered by Ag-NPs. Taken together, our findings suggested that oral administration of Ag-NPs induced neurotoxicity in rats and rutin mitigates these effects.
在过去几十年中，由于银纳米粒子（Ag-NPs）在纳米尺度上的独特性质及其在最近推出的许多商业产品中的广泛应用，对其进行毒理学研究已成为一个热门话题。本研究旨在探讨 Ag-NPs 对神经递质的毒性作用，特别强调了 Ag-NPs 与芦丁同时给药对实验大鼠的影响。Ag-NPs 经口给予大鼠可诱导脑氧化应激，显著改变神经递质和氨基酸。此外，兴奋性氨基酸 N-甲基-D-天冬氨酸（NMDA）受体、单胺氧化酶（MAO-A、MAO-B）和金属硫蛋白-III（MT-III）的转录水平在 Ag-NPs 中毒大鼠中显著升高。此外，组织学检查显示星形胶质细胞增生和神经元脱髓鞘，伴有神经元变性和空泡形成。值得注意的是，芦丁的口服给药可逆转 Ag-NPs 引发的毒性作用。总之，我们的研究结果表明，Ag-NPs 的经口给予可诱导大鼠的神经毒性，而芦丁可减轻这些作用。
