Gao Yu, Liu Qian, Wang Min, Zhao Guihua, Jiang Yong, Malin Gill, Gong Zheng, Meng Xue, Liu Zhaoyang, Lin Tongtong, Li Yutong, Shao Hongbing
College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
Curr Microbiol. 2017 Jul;74(7):821-826. doi: 10.1007/s00284-017-1251-9. Epub 2017 Apr 20.
A novel marine Alteromonas gracilis siphovirus, phage PB15, was isolated from the surface water of the Yellow Sea in August 2015. It has a head diameter of 58 ± 5 nm head and a contractile tail approximately 105 ± 10 nm in length, and overall, the morphology suggests that PB15 belongs to the family Siphoviridae. PB15 phage is stable at over the temperature range 0-60 °C. The best MOI of these phage was 0.1, and infectivity decreased above 60 °C. The results suggest that phage is stable at pH value ranging between 3.0 and 11.0. Chloroform test shows that PB15 is not a lipid-containing phage. A one-step growth curve with a strain of A. gracilis gave a latent period of 16 min and rise period of 24 min and burst size of 60 PFU/cell. Genomic analysis of PB15 reveals a genome size of 37,333 bp with 45.52% G+C content, and 61 ORFs. ORF sequences accounted for 30.36% of the genome sequence. There is no obvious similarity between PB15 and other known phages by genomic comparison using the BLASTN tool in the NCBI database.
2015年8月，从黄海表层水体中分离出一种新型海洋纤细交替单胞菌肌尾噬菌体PB15。它的头部直径为58±5纳米，收缩尾长约105±10纳米，总体而言，其形态表明PB15属于肌尾噬菌体科。PB15噬菌体在0至60°C的温度范围内稳定。这些噬菌体的最佳感染复数为0.1，60°C以上感染力下降。结果表明，噬菌体在pH值3.0至11.0范围内稳定。氯仿试验表明PB15不是含脂质噬菌体。用一株纤细交替单胞菌进行的一步生长曲线显示，潜伏期为16分钟，上升期为24分钟，裂解量为60个噬菌斑形成单位/细胞。PB15的基因组分析显示，基因组大小为37333碱基对，G+C含量为45.52%，有61个开放阅读框。开放阅读框序列占基因组序列的30.36%。使用NCBI数据库中的BLASTN工具进行基因组比较，PB15与其他已知噬菌体之间没有明显的相似性。
