School of Bioscience and Biopharmaceutics, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, China.
College of Pharmacy, Guangdong Pharmaceutical University, 510006 Guangzhou, Guangdong, China.
J Ethnopharmacol. 2020 Sep 15;259:112953. doi: 10.1016/j.jep.2020.112953. Epub 2020 May 11.
Danggui Buxue Tang (DBT) has been used to promote hematopoiesis and relieve myelosuppression in China. Antibiotics can cause myelosuppression through gut microbiota disorders.
This study aims to explore the way of DBT to alleviate the metabolic disorder caused by antibiotics.
In this study, 16S rRNA sequencing was used to detect the change of gut microbiota, metabolomics to analyze the change of metabolites. Correlation analysis was used to establishment the correlation between gut microbiota and metabolites. PICRUST 2 was used to predict the function of gut microbiota.
Results showed that eighty-two genera of gut microbiota were affected by antibiotic, while twelve were significantly restored after DBT. Seventy-four potential metabolites were significantly different from the antibiotics and DBT. We found significant recovery by the Bacteroides and Rikenellaceae RC9 after DBT. The metabolic pathways influenced by the antibiotic treatment included primary and secondary bile biosynthesis, etc. The metabolic pathways that could be restored after DBT included the primary and secondary bile acid biosynthesis pathway, etc. Through correlation analysis, we found a correlation between the Bacteroides, Rikenellaceae_RC9_gut_group and other potential differential metabolisms such as those of taurodeoxycholic acid, N-phenylacetyl glycine, etc. The functional prediction showed that the biosynthesis of primary bile acid, secondary bile acid was significantly affected.
DBT can restore the gut and reverse the metabolic disorder caused by antibiotics through Bacteroides, and it provides a new medical idea regarding the gut microbiota balance.
当归补血汤（DBT）已在中国被用于促进造血和缓解骨髓抑制。抗生素可通过肠道微生物群紊乱引起骨髓抑制。
本研究旨在探讨 DBT 缓解抗生素引起的代谢紊乱的途径。
在这项研究中，使用 16S rRNA 测序来检测肠道微生物群的变化，代谢组学来分析代谢物的变化。使用相关分析来建立肠道微生物群和代谢物之间的相关性。PICRUST 2 用于预测肠道微生物群的功能。
结果表明，抗生素影响了 82 个肠道微生物群属，而 DBT 后有 12 个属得到显著恢复。有 74 种潜在代谢物与抗生素和 DBT 显著不同。DBT 后，拟杆菌和理研菌科 RC9 显著恢复。受抗生素处理影响的代谢途径包括初级和次级胆酸生物合成等。DBT 后可恢复的代谢途径包括初级和次级胆酸生物合成途径等。通过相关分析，我们发现拟杆菌、理研菌科 RC9 肠道群与 taurodeoxycholic acid、N-phenylacetyl glycine 等其他潜在差异代谢物之间存在相关性。功能预测显示，初级胆汁酸、次级胆汁酸的生物合成受到显著影响。
DBT 可以通过拟杆菌恢复肠道并逆转抗生素引起的代谢紊乱，为肠道微生物群平衡提供了新的医学思路。
