Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 1450 NW 10th Avenue, Miami, FL, 33136, USA.
Department of Cell Biology, University of Miami Leonard M. Miller School of Medicine, Miami, FL, 33136, USA.
Inflammopharmacology. 2018 Feb;26(1):157-170. doi: 10.1007/s10787-017-0349-9. Epub 2017 Apr 20.
Cinnamomum verum (CV), also known as 'Dalchini', is the dry bark of the Cinnamomum verum (L.) plant, and has been used as a traditional Pakistani medicine to alleviate pain and inflammation in patients suffering from arthritic rheumatism. It contains alkaloids, triterpenes, Cinnamaldehyde and other volatile oils. The aim of the present study was to investigate the underlying biological effect of ethyl alcohol (EtOH) and methyl alcohol (MeOH) extracts from CV on collagen type-II induced arthritic (CIA) mice.
Gas chromatography mass spectrophotometry was used to perform fingerprinting identification of the EtOH and MeOH extracts. CIA mice model was established by subdermal injections of type-II bovine collagen (CII) on the 1st, 8th and 14th day of the experiment. Ethyl alcohol extract and methyl alcohol extract (1 mg/KgBW, 2 mg/KgBW and 4 mg/KgBW), was orally administered from the 15th day onwards for 2 weeks. Progression of oedema and joint inflammation was measured in the paws using a digital Vernier calliper every 3 days from day 1 till the end of the experiment. The oxidative scavenging ability of cinnamaldehyde was evaluated using a DPPH assay. Similarly, the nitrogen free radical (NOS) production of isolated lymphocytes was evaluated using Greiss's method. The spleen index was calculated and knee joint changes were observed by histopathological sectioning. Western blot analysis was performed on peripheral blood derived serum for CII, CAPN1, TNFα and NFATc3.
Extracts were shown to be enriched in trans-cinnamaldehyde and its analogues. Extracts showed good ameliorative effects (p < 0.05) after day 2 of treatment. A greater therapeutic role was observed for the 4 mg/kgBW dosage of the methanolic extract (p < 0.01). Swelling in the spleen was greatly reduced along with the generation of free radicals by lymphocytes, post treatment. There was also an inhibitory role by the extracts on NFATc3 (p < 0.05), TNF-Alpha (p < 0.05), CAII (p < 0.05) and mCalpain (p < 0.05) all proteins involved in RA.
In this study, it has been demonstrated that administration of CV has a therapeutic potential on CIA. The data suggest that CV could have a potential role in the treatment of RA patients.
肉桂（CV），也被称为“Dalchini”，是肉桂树（Cinnamomum verum（L.））的干燥树皮，已被用作巴基斯坦传统药物，以减轻关节炎风湿患者的疼痛和炎症。它含有生物碱、三萜、肉桂醛和其他挥发油。本研究的目的是研究 CV 的乙醇（EtOH）和甲醇（MeOH）提取物对胶原 II 诱导的关节炎（CIA）小鼠的潜在生物学作用。
气相色谱质谱联用仪用于对 EtOH 和 MeOH 提取物进行指纹鉴定。通过在实验的第 1、8 和 14 天在皮下注射 II 型牛胶原蛋白（CII）来建立 CIA 小鼠模型。从第 15 天开始，口服给予乙醇提取物和甲醇提取物（1mg/KgBW、2mg/KgBW 和 4mg/KgBW），持续 2 周。从第 1 天开始，每隔 3 天使用数字游标卡尺测量爪子中的水肿和关节炎症的进展，直到实验结束。使用 DPPH 测定法评估肉桂醛的抗氧化清除能力。同样，使用 Greiss 法评估分离淋巴细胞的无氮自由基（NOS）产生。计算脾指数并通过组织病理学切片观察膝关节变化。对来源于外周血的血清进行 Western blot 分析，以检测 CII、CAPN1、TNFα 和 NFATc3。
提取物中富含反式肉桂醛及其类似物。治疗后第 2 天，提取物表现出良好的改善作用（p<0.05）。甲醇提取物的 4mg/kgBW 剂量表现出更大的治疗作用（p<0.01）。治疗后，脾肿胀和淋巴细胞产生的自由基大大减少。提取物还对 NFATc3（p<0.05）、TNF-Alpha（p<0.05）、CAII（p<0.05）和 mCalpain（p<0.05）均有抑制作用，这些蛋白均参与 RA。
在这项研究中，已经证明 CV 的给药对 CIA 具有治疗潜力。数据表明，CV 可能在治疗 RA 患者方面发挥作用。
