Pharmacology Department, Faculty of Medicine, Dongola University, Dongola, Sudan.
Toxicology & Pharmacology Department Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Environ Sci Pollut Res Int. 2020 Jan;27(3):2829-2843. doi: 10.1007/s11356-019-07164-w. Epub 2019 Dec 13.
This study aimed to evaluate the protective role of encapsulated cinnamon oil emulsion (COE) in whey protein concentrate (WPC) against the disturbance in lipid profile, oxidative stress markers, and gene expression in streptozotocin (STZ)-treated rats. COE was analyzed using GC-MS, and the emulsion was prepared and characterized. In the in vivo study, six groups of male rats were treated orally for 4 weeks, including the control group, the group treated with STZ (D-rats), the groups received a low or high dose of COE (200 or 400 mg/kg B.w.), and the D-rats groups received COE at the low or high dose. Blood and tissue samples were collected after the end of the treatment period for biochemical, genetical, and histological analyses. The GC-MS results revealed that the major components of the oil were cinnamaldehyde, 1,8 cineole, acetic acid, 1,7,7-trimethylbicyclo[2.2.1]hept2yl ester, α-Pinene, and α-Terpineol. The size, zeta potential, and polydispersity index (PDI) of COE were 240 ± 1.03 nm, - 7.09 ± 0.42, and 0.36, respectively. The in vivo results revealed that COE at the two tested doses improved the levels of glucose, insulin, amylase, lipid profile, hepatic MDA, SOD, and GSH. COE also downregulated hepatic GLU2, FAS, SREBP-1c, and PEPCK gene expression and upregulated IGF-1 mRNA expression in diabetic rats in a dose-dependent manner. Moreover, COE improved and the histological picture of the liver and pancreas. It could be concluded that COE overcomes the disturbances in biochemical, cytological, and histopathological changes in D-rats via the enhancement of antioxidant capacity; reduces the oxidative stress; modulates the concerned gene expression; and may be promising to develop new drugs for diabetic treatment.
本研究旨在评估包封肉桂油乳液（COE）对乳清蛋白浓缩物（WPC）在链脲佐菌素（STZ）处理大鼠脂质谱、氧化应激标志物和基因表达紊乱的保护作用。使用 GC-MS 对 COE 进行分析，并制备和表征乳液。在体内研究中，将六组雄性大鼠口服治疗 4 周，包括对照组、STZ 处理组（D 大鼠）、低或高剂量 COE（200 或 400mg/kg BW）组和 D 大鼠组接受低或高剂量 COE。治疗期结束后采集血液和组织样本进行生化、遗传和组织学分析。GC-MS 结果表明，该油的主要成分是肉桂醛、1,8-桉叶素、乙酸、1,7,7-三甲基双环[2.2.1]庚-2-基酯、α-蒎烯和α-萜品烯。COE 的大小、Zeta 电位和多分散指数（PDI）分别为 240±1.03nm、-7.09±0.42 和 0.36。体内结果表明，COE 在两种测试剂量下均能提高葡萄糖、胰岛素、淀粉酶、脂质谱、肝 MDA、SOD 和 GSH 的水平。COE 还能下调糖尿病大鼠肝 GLU2、FAS、SREBP-1c 和 PEPCK 基因表达，并呈剂量依赖性地上调 IGF-1mRNA 表达。此外，COE 改善了肝和胰腺的组织学图片。综上所述，COE 通过增强抗氧化能力克服了 D 大鼠生化、细胞学和组织病理学变化的紊乱；降低氧化应激；调节相关基因表达；并可能有望开发用于糖尿病治疗的新药。
