Aix-Marseille University, Centre National de la Recherche Scientifique, Information Génomique &Structurale, Unité Mixte de Recherche 7256 (Institut de Microbiologie de la Méditerranée, FR3479), 13288 Marseille Cedex 9, France.
Université Grenoble Alpes, BIG-BGE, F-38000 Grenoble, France.
Nat Commun. 2017 Apr 21;8:15087. doi: 10.1038/ncomms15087.
Acanthamoeba are infected by a remarkable diversity of large dsDNA viruses, the infectious cycles of which have been characterized using genomics, transcriptomics and electron microscopy. Given their gene content and the persistence of the host nucleus throughout their infectious cycle, the Marseilleviridae were initially assumed to fully replicate in the cytoplasm. Unexpectedly, we find that their virions do not incorporate the virus-encoded transcription machinery, making their replication nucleus-dependent. However, instead of delivering their DNA to the nucleus, the Marseilleviridae initiate their replication by transiently recruiting the nuclear transcription machinery to their cytoplasmic viral factory. The nucleus recovers its integrity after becoming leaky at an early stage. This work highlights the importance of virion proteomic analyses to complement genome sequencing in the elucidation of the replication scheme and evolution of large dsDNA viruses.
棘阿米巴可被多种大型双链 DNA 病毒感染，这些病毒的感染周期已通过基因组学、转录组学和电子显微镜进行了描述。鉴于它们的基因组成和其在整个感染周期中宿主核的持续存在，马赛病毒科最初被假定在细胞质中完全复制。出乎意料的是，我们发现它们的病毒粒子不包含病毒编码的转录机制，这使得它们的复制依赖于细胞核。然而，马赛病毒科并没有将其 DNA 递送到细胞核，而是通过短暂地将核转录机制招募到其细胞质病毒工厂来启动复制。在早期阶段核变得有渗漏性后，核恢复其完整性。这项工作强调了病毒粒子蛋白质组分析在阐明大型双链 DNA 病毒的复制方案和进化方面补充基因组测序的重要性。
