Cardiology Group, Health Research Institute, University Clinical Hospital of Santiago de Compostela, Santiago de Compostela, Spain.
CiberCV Madrid, Spain.
Obesity (Silver Spring). 2017 Jun;25(6):1042-1049. doi: 10.1002/oby.21832. Epub 2017 Apr 21.
Epicardial adipose tissue (EAT) in coronary artery disease is insulin resistant and has a proinflammatory profile. This study examined the regulation of EAT by exogenous omentin and its consequence on vascular cells.
Stromal vascular cells (SC) of EAT and subcutaneous adipose tissue (SAT) from patients who underwent heart surgery were cultured and exposed to adipogenic factors with or without omentin. Proinflammatory cytokine regulation by omentin was analyzed in SC and mature adipocytes. Glucose uptake by EAT and SAT explants was determined after insulin, omentin, or combined treatment. Human vascular cells were exposed to secretomes of SC, with and without omentin treatment. Migration of smooth muscle cells and expression of adhesion molecules were determined by wound healing or real-time polymerase chain reaction, respectively.
Omentin treatment raised adipogenesis-induced adiponectin levels on SC of EAT and reduced TNF-α expression levels (0.58 ± 0.14-fold change; P = 0.034) in mature adipocytes. Omentin improved the insulin activity of EAT and SAT explants from cardiovascular disease patients. Finally, secretomes of SC under omentin treatment reduced the migration of smooth muscle cells.
Exogenous omentin might support a cardioprotective role through its effect on EAT regarding glucose uptake, anti-inflammatory response, and its paracrine role on smooth muscle cells.
冠状动脉疾病患者的心外膜脂肪组织（EAT）存在胰岛素抵抗，并呈现促炎状态。本研究旨在探讨外源性网膜素对 EAT 的调节作用及其对血管细胞的影响。
对接受心脏手术的患者的 EAT 和皮下脂肪组织（SAT）的基质血管细胞（SC）进行培养，并在存在或不存在网膜素的情况下使其暴露于脂肪生成因子中。分析网膜素对 SC 和成熟脂肪细胞中促炎细胞因子的调节作用。在胰岛素、网膜素或联合处理后，测定 EAT 和 SAT 外植体的葡萄糖摄取情况。用 SC 的分泌组和经网膜素处理的分泌组分别暴露于血管细胞。通过划痕愈合或实时聚合酶链反应分别测定平滑肌细胞的迁移和粘附分子的表达。
网膜素处理可提高 EAT 的 SC 中脂肪生成诱导的脂联素水平，并降低成熟脂肪细胞中的 TNF-α表达水平（0.58±0.14 倍变化；P=0.034）。网膜素可改善心血管疾病患者的 EAT 和 SAT 外植体的胰岛素活性。最后，网膜素处理的 SC 分泌组可减少平滑肌细胞的迁移。
外源性网膜素可能通过其对 EAT 的葡萄糖摄取、抗炎反应以及对平滑肌细胞的旁分泌作用，发挥其对 EAT 的心脏保护作用。
