Mena-Bueno Sara, Atanasova Miroslava, Fernández-Trasancos Ángel, Paradela-Dobarro Beatriz, Bravo Susana B, Álvarez Ezequiel, Fernández Ángel L, Carrera Iván, González-Juanatey José R, Eiras Sonia
Cardiology Group, Health Research Institute, University Clinical Hospital of Santiago de Compostela, Spain.
ANFACO-CECOPESCA, Vigo, Spain.
Food Funct. 2016 Feb;7(2):953-63. doi: 10.1039/c5fo01246e.
epicardial adipose tissue (EAT) from patients with coronary artery disease (CAD) contains higher levels of inflammatory proteins and lower adiponectin levels than subcutaneous adipose tissue (SAT), enhancing the progression of atherosclerosis. Since products from sea cucumber have anti-inflammatory properties, we investigated its effect on EAT, SAT and endothelial cells.
stromal cells or explants from EAT and SAT were obtained from patients with cardiovascular disease. Extracts were obtained after hydrolysis by food-grade enzymes at different times. Proteins were identified by LC-MALDI mass spectrometry. Adipogenesis and adiponectin induction were determined on stromal cells in the presence/absence of extracts. The bioavailability of the extracts was tested on a Caco-2 cell culture model in vitro. The bioavailable fraction was probed on endothelial cells and EAT or SAT explants. Vascular cell adhesion protein (VCAM-1), intercellular adhesion molecule (ICAM-1), IL-6 and adiponectin were determined by real time polymerase chain reaction (RT-PCR).
our results showed that H. forskali and P. tremulus extracts contained compounds with anti-oxidant and anti-inflammatory properties. The bioavailable fraction of P. tremulus reduced VCAM-1 (p < 0.01) and IL-6 (p < 0.05) expression levels in endothelial cells while bioavailable compounds from H. forskali decreased ICAM-1 expression in SAT (p < 0.05). No effect was observed on EAT.
these results suggest that sea cucumber extracts might be used for the prevention of endothelial cells and SAT inflammation.
与皮下脂肪组织（SAT）相比，冠心病（CAD）患者的心外膜脂肪组织（EAT）含有更高水平的炎症蛋白和更低水平的脂联素，这会促进动脉粥样硬化的进展。由于海参产品具有抗炎特性，我们研究了其对EAT、SAT和内皮细胞的影响。
从心血管疾病患者中获取EAT和SAT的基质细胞或外植体。在不同时间用食品级酶水解后获得提取物。通过液相色谱 - 基质辅助激光解吸电离质谱法鉴定蛋白质。在有/无提取物存在的情况下，在基质细胞上测定脂肪生成和脂联素诱导情况。在体外Caco - 2细胞培养模型上测试提取物的生物利用度。在内皮细胞以及EAT或SAT外植体上检测生物可利用部分。通过实时聚合酶链反应（RT - PCR）测定血管细胞黏附蛋白（VCAM - 1）、细胞间黏附分子（ICAM - 1）、白细胞介素 - 6（IL - 6）和脂联素。
我们的结果表明，刺参和绿刺参提取物含有具有抗氧化和抗炎特性的化合物。绿刺参的生物可利用部分降低了内皮细胞中VCAM - 1（p < 0.01）和IL - 6（p < 0.05）的表达水平，而刺参的生物可利用化合物降低了SAT中ICAM - 1的表达（p < 0.05）。对EAT未观察到影响。
这些结果表明，海参提取物可能用于预防内皮细胞和SAT炎症。
