N-叔丁基-α-苯基硝酮（PBN）用于离体灌注心脏自旋捕获实验的能力：初步研究。
Ability of N-tert-butyl alpha phenylnitrone (PBN) to be used in isolated perfused heart spin trapping experiments: preliminary studies.
作者信息
Charlon V, de Leiris J
机构信息
Laboratoire de Physiopathologie du Métabolisme Cardiaque, Université Scientifique Technologique et Médicale de Grenoble, France.
出版信息
Basic Res Cardiol. 1988 May-Jun;83(3):306-13. doi: 10.1007/BF01907364.
The aim of this study was to investigate the possibility of oxygen-free radical spin trapping with PBN, in models of isolated perfused hearts. Preliminary studies reported here demonstrate that (i) PBN may be precisely measured with UV spectroscopy, (ii) commercially available PBN does not show any ESR signal, (iii) PBN does not trap significant amounts of free radicals in a perfusion medium oxygenated for at least 3 h, and (iv) when added at 15 or 56 mM in the perfusion medium, PBN is a highly toxic compound, whereas no toxic effect was observed with 3mM-containing perfusate.
本研究的目的是在离体灌注心脏模型中研究用PBN进行无氧自由基自旋捕获的可能性。此处报告的初步研究表明：（i）可用紫外光谱精确测量PBN；（ii）市售PBN未显示任何电子自旋共振信号；（iii）PBN在充氧至少3小时的灌注介质中不会捕获大量自由基；（iv）当在灌注介质中添加15或56 mM的PBN时，它是一种剧毒化合物，而含3 mM的灌注液未观察到毒性作用。
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