Payment P, Trudel M
Centre de recherche en virologie, Institut Armand-Frappier, Ville de Laval, Qué., Canada.
Can J Microbiol. 1988 Mar;34(3):271-2. doi: 10.1139/m88-049.
Wound fiberglass depth cartridge filters (25.4 cm) with a nominal porosity of 1 micron were used to concentrate viruses from large volumes of surface water. They were found to be an excellent, less expensive alternative to the 0.2-micron pleated cartridge filters normally used for the concentration of enteric viruses from water. More than 99% of experimentally seeded poliovirus was adsorbed to these filters when the pH of the water was adjusted to pH 3.5 and aluminium chloride was added to a final concentration of 0.001 M, as recommended for electronegative filters. In comparative recovery of indigenous viruses from river water, similar results were obtained with two 1-micron or a 3-microns + 0.2-micron filter combination. The cost of the two 1-micron filters is about Can. $26, while it is about Can. $58 for the other combination.
使用标称孔隙率为1微米的伤口玻璃纤维深层滤芯（25.4厘米）从大量地表水中浓缩病毒。结果发现，对于通常用于从水中浓缩肠道病毒的0.2微米褶式滤芯而言，它们是一种出色且成本较低的替代品。按照电负性过滤器的推荐，当将水的pH值调节至pH 3.5并添加氯化铝至最终浓度为0.001 M时，超过99%的实验接种脊髓灰质炎病毒吸附到这些过滤器上。在从河水中比较回收本地病毒时，使用两个1微米或一个3微米 + 0.2微米过滤器组合可获得类似结果。两个1微米过滤器的成本约为26加元，而另一种组合的成本约为58加元。
