Audurier A, Pardon P, Marly J, Lantier F
Ann Microbiol (Paris). 1980 Jul-Aug;131B(1):47-57.
Swiss mice were infected with two Listeria strains: L. monocytogenes strain 10401, serovar 4b, and L. innocua strain 390, serovar 6a. Bacteria were inoculated by intravenous, subcutaneous or oral routes, and then enumerated in the spleen. The splenic infection was studied comparatively for these three inoculation routes with both strains. Strain 390 caused a splenic colonization only after intravenous inoculation. For the 10401 strain, the peak of infection appeared on the 3rd day after inoculation; the intravenous route was the most efficient to kill mice, the subcutaneous one the most efficient to obtain a reproductible sublethal infection; the oral route infected regularly only with doses higher than 10(7) bacteria. A splenomegaly appeared only in mice infected with strain 10401. Estimation of Listeria strain pathogenicity depended more on the measure of bacterial count in the spleen on the 3rd day of infection than on LD50. To be able to compare quantitatively the pathogenicity of L. monocytogenes and L. innocua, it seemed impossible to use only one dose of bacteria and to inoculate through one route.
单核细胞增生李斯特菌10401菌株，血清型4b，以及无害李斯特菌390菌株，血清型6a。通过静脉内、皮下或口服途径接种细菌，然后在脾脏中计数。比较研究了这两种菌株通过这三种接种途径引起的脾脏感染情况。390菌株仅在静脉内接种后才引起脾脏定植。对于10401菌株，感染高峰出现在接种后第3天；静脉途径最易导致小鼠死亡，皮下途径最易获得可重复的亚致死感染；口服途径只有在细菌剂量高于10(7)时才会经常引起感染。只有感染10401菌株的小鼠出现了脾肿大。评估李斯特菌菌株的致病性更多地取决于感染第3天脾脏中细菌计数的测量，而不是半数致死剂量（LD50）。为了能够定量比较单核细胞增生李斯特菌和无害李斯特菌的致病性，似乎不可能仅使用一种细菌剂量并通过一种途径进行接种。
