Mehta Shibha, Verma Promila, Tikku Aseem Prakash, Chandra Anil, Bains Rhythm, Banerjee Gopa
Department of Conservative Dentistry and Endodontics, Faculty of Dental Sciences, King George's Medical University, Lucknow, Uttar Pradesh, India.
Department of Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, India.
Eur J Dent. 2017 Jan-Mar;11(1):53-57. doi: 10.4103/ejd.ejd_159_16.
The objective of this study is to compare the antimicrobial efficacy of triple antibiotic paste (TAP) and a proton pump inhibitor (PPI) (omeprazole) in combination with calcium hydroxide (CH) against and .
and were subcultured and inoculated at 37° overnight and were treated with different dilutions of TAP, 25 μg/ml (Group 1), CH (Group 2, control), CH 16 mg/ml + omeprazole 2 mg/ml (Group 3a) (CH 16 mg/ml + omeprazole 4 mg)/ml (Group 3b) for 24, 48, and 72 h in sterile uncoated 96-well microtiter plates. Minimum concentration at which the medicaments produced least optical density was determined using ELISA reader (ELx 808 BioTek Inc., USA) device set at optical density of 630 nm. Results were analyzed statistically by one-way analysis of variance followed by Tukey's multiple comparison tests. The significance level was set at 0.05.
Mean concentration (irrespective of time) for TAP at which mean minimum optical density was recorded at 1.25 μg/ml (1:20 dilution) and 25 μg/ml (0 dilution) against and , respectively. Least optical density for CH plus PPI group was obtained 1.6 μg/ml (1:10 dilution) and 16 μg/ml (0 dilution) for and , respectively. However, CH alone showed a weaker antimicrobial action against either of the strains even at full concentration.
PPI enhanced the antibacterial efficacy of CH against and . However, TAP showed the best antibacterial property followed by CH plus PPIs against both the selected strains.
本研究旨在比较三联抗生素糊剂（TAP）以及质子泵抑制剂（PPI）（奥美拉唑）联合氢氧化钙（CH）对[具体菌种1]和[具体菌种2]的抗菌效果。
将[具体菌种1]和[具体菌种2]进行传代培养，并在37℃下过夜接种，然后在无菌无涂层的96孔微量滴定板中，用不同稀释度的TAP（25μg/ml，第1组）、CH（第2组，对照组）、CH 16mg/ml + 奥美拉唑2mg/ml（第3a组）、CH 16mg/ml + 奥美拉唑/4mg/ml（第3b组）处理24、,48和72小时。使用酶标仪（美国BioTek公司ELx 808）在630nm光密度下测定药物产生最小光密度的最低浓度。结果采用单因素方差分析，随后进行Tukey多重比较检验进行统计学分析。显著性水平设定为0.05。
TAP在分别针对[具体菌种1]和[具体菌种2]时，记录到平均最小光密度的平均浓度（与时间无关）分别为1.25μg/ml（1:20稀释）和25μg/ml（0稀释）。CH加PPI组针对[具体菌种1]和[具体菌种2]分别在1.6μg/ml（1:10稀释）和16μg/ml（0稀释）时获得最小光密度。然而，单独的CH即使在全浓度下对任何一种菌株的抗菌作用都较弱。
PPI增强了CH对[具体菌种1]和[具体菌种2]的抗菌效果。然而，TAP显示出最佳的抗菌性能，其次是CH加PPI对两种选定菌株的抗菌性能。
