Department of Endodontic, Faculty of Dentistry, University of October 6, Cairo, Egypt.
Department of Dental Biomaterials, Faculty of Dentistry, University of October 6, Cairo, Egypt.
Eur Endod J. 2021 Apr;6(1):82-89. doi: 10.14744/eej.2020.29292. Epub 2021 Mar 12.
The aim of this study is to compare the cytotoxicity of triple antibiotic paste (TAP) with an antiinflammatory drug (TAP+Catafast-TAPC) in nano and regular formulations versus calcium hydroxide as intracanal medicaments.
The TAPC drugs extraction were made in cell culture media MEM-E (Eagle's minimal essential medium) using concentration of 10 mg/mL of each sample for seven days. Inhibitory concentrations (IC50 values) were determined for each extract. A human fibroblasts cell line was used to evaluate the cytotoxicity of different concentrations (10, 0.625 and 0.07 mg/mL) using MTT essay. The cell viability was measured after 24 h, 48 h and 7 days for all concentrations of the drugs. Flow cytometry analysis was carried out to identify the effect of materials on apoptosis/necrosis. Statistical analysis for the obtained results was done by one-way ANOVA.
The results revealed that cell viability was inversely proportional to the duration of treatment in all of the groups. Calcium hydroxide (Control group) demonstrated a significantly greater cytotoxic effect, followed by Nano Triple Antibiotic Paste with Catafast as an anti-inflamatory drug (Nano TAPC), while Triple Antibiotic Paste with Catafast (TAPC) had the least cytotoxic effect. Nano TAPC has the greatest apoptotic value, while TAPC had the least when compared with the reference group, with no significant difference between groups (P<0.05).
The cytotoxic effect of Nano TAPC was lower than that of calcium hydroxide and higher than that of TAPC. Although Nano TAPC has the highest apoptotic value when compared to TAPC and calcium hydroxide but still there is no statistically significant difference between them.
本研究旨在比较三种抗生素糊剂（TAP）与含有抗炎药物的 TAP（TAP+Catafast-TAPC）在纳米和常规制剂与氢氧化钙作为根管内药物的细胞毒性。
使用浓度为 10 mg/mL 的每种样品在细胞培养基 MEM-E（Eagle 的最小必需培养基）中提取 TAPC 药物 7 天。测定每种提取物的抑制浓度（IC50 值）。使用 MTT 试验评估不同浓度（10、0.625 和 0.07 mg/mL）的不同浓度（10、0.625 和 0.07 mg/mL）对不同浓度（10、0.625 和 0.07 mg/mL）的细胞毒性。在所有药物浓度下，在 24 小时、48 小时和 7 天时测量细胞活力。通过流式细胞术分析来确定材料对细胞凋亡/坏死的影响。对获得的结果进行单向方差分析的统计分析。
结果表明，在所有组中，细胞活力与治疗时间成反比。氢氧化钙（对照组）表现出明显更大的细胞毒性作用，其次是含有抗炎药物的纳米三种抗生素糊剂（纳米 TAPC），而含有抗炎药物的 TAPC（TAPC）的细胞毒性作用最小。纳米 TAPC 的凋亡值最大，而 TAPC 与参考组相比最小，但组间无显著差异（P<0.05）。
纳米 TAPC 的细胞毒性作用低于氢氧化钙，高于 TAPC。尽管纳米 TAPC 的凋亡值与 TAPC 和氢氧化钙相比最高，但它们之间仍无统计学差异。
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