Mavromoustakis C T, Witiak D T, Hughes J H
Department of Medical Microbiology and Immunology, College of Medicine, Ohio State University.
J Virol Methods. 1988 Jun;20(2):95-100. doi: 10.1016/0166-0934(88)90142-5.
The effect of rolling on the number of herpes simplex virus (HSV) foci and yield of infectious virus was examined for cultures inoculated with clinical specimens. Inoculated cultures were either rolled at 2 revolutions per minute (rpm) or held stationary. Cultures that were rolled at 2 rpm showed a significantly greater number of foci and plaque forming units (PFU) over stationary cultures. The geometric mean fold increase in PFU between rolled and stationary cultures was 8-fold. Rolling of inoculated cultures should be used in the clinical virology laboratory to aid in the rapid detection of HSV.
对于接种临床标本的培养物，研究了滚动对单纯疱疹病毒（HSV）病灶数量和感染性病毒产量的影响。接种后的培养物要么以每分钟2转（rpm）的速度滚动，要么保持静止。以2 rpm滚动的培养物比静止培养物显示出明显更多的病灶和空斑形成单位（PFU）。滚动培养物与静止培养物之间PFU的几何平均增加倍数为8倍。临床病毒学实验室应采用接种培养物滚动的方法，以帮助快速检测HSV。
