Mavromoustakis C T, Witiak D T, Hughes J H
Department of Medical Microbiology and Immunology, College of Medicine, Columbus, Ohio.
J Clin Microbiol. 1988 Nov;26(11):2328-31. doi: 10.1128/jcm.26.11.2328-2331.1988.
We examined the effect of high-speed rolling on herpes simplex virus replication. Inoculated cultures were rolled at 2, 96, or 383 rpm, while stationary cultures served as controls. At 24 h, inoculated cultures rolled at 96 rpm had a 6.8-fold increase in foci when compared with stationary cultures (P less than 0.01) and a 2.8-fold increase over cultures rolled at 2 rpm (P less than 0.05). Cultures rolled at 2 rpm had a 2.4-fold increase in foci over stationary cultures (P less than 0.05). Viral yield results correlated with focus results. Significantly more virus was present in cultures rolled at 96 rpm (7.3-fold) than in stationary cultures. Cultures rolled at 2 rpm produced 2.9-fold more virus than stationary cultures (P less than 0.05). Of 37 cultures rolled at 96 rpm, 33 (89%) were cytopathic effect positive at 96 h, while 18 of 40 (45%) were positive at 2 rpm and only 2 of 37 (5%) were positive for stationary cultures (P less than 0.01). Cultures rolled at 96 rpm produced maximum viral yields 2 days sooner than stationary cultures. Rolling of inoculated cultures should be used in the clinical laboratory to aid in the rapid detection of herpes simplex virus.
我们研究了高速旋转对单纯疱疹病毒复制的影响。对接种后的培养物分别以2、96或383转/分钟的速度进行旋转，而静止培养物作为对照。24小时时，与静止培养物相比，以96转/分钟旋转的接种培养物的病灶增加了6.8倍（P小于0.01），比以2转/分钟旋转的培养物增加了2.8倍（P小于0.05）。以2转/分钟旋转的培养物的病灶比静止培养物增加了2.4倍（P小于0.05）。病毒产量结果与病灶结果相关。以96转/分钟旋转的培养物中存在的病毒明显多于静止培养物（7.3倍）。以2转/分钟旋转的培养物产生的病毒比静止培养物多2.9倍（P小于0.05）。在37个以96转/分钟旋转的培养物中，33个（89%）在96小时时出现细胞病变效应阳性，而在40个以2转/分钟旋转的培养物中有18个（45%）阳性，在37个静止培养物中只有2个（5%）阳性（P小于0.01）。以96转/分钟旋转的培养物比静止培养物提前2天产生最大病毒产量。临床实验室应采用对接种培养物进行旋转的方法来辅助快速检测单纯疱疹病毒。
