Hughes J H, Hamparian V V, Mavromoustakis C T
Department of Medical Microbiology and Immunology, College of Medicine, Ohio State University, Columbus 43210.
J Clin Microbiol. 1989 Dec;27(12):2884-6. doi: 10.1128/jcm.27.12.2884-2886.1989.
Specimens submitted for diagnosis of herpes simplex virus infections were inoculated into shell vials and conventional culture tubes. Inoculated culture tubes were incubated with rolling at 96 rpm. Immunoperoxidase (IP) staining and cytopathic effects (CPE) were used to detect positive cultures. At 24 h, 42 (53%) of the rolled cultures were positive for CPE, while only 16 (21%) of the shell vials were CPE positive (P less than 0.01). No difference in sensitivity was seen between rolled and shell vial cultures that were inoculated with high-titered viral preparations and IP stained at 16 h. However, when low-titered preparations were used, 39 of 41 (95%) were IP positive by the high-speed roller method at 64 h postinoculation, while only 24 of 41 (58%) were IP positive with shell vials (P less than 0.01). These results indicate that high-speed roller method at 64 h postinoculation, while only 24 of 41 (58%) were IP positive with shell vials (P less than 0.01). These results indicate that high-speed rolling is better than the shell vial technique for the detection of herpes simplex virus by IP staining.
提交用于单纯疱疹病毒感染诊断的标本被接种到空斑试验管和传统培养管中。接种后的培养管以96转/分钟的转速滚动培养。免疫过氧化物酶（IP）染色和细胞病变效应（CPE）用于检测阳性培养物。24小时时，42份（53%）滚动培养的标本CPE呈阳性，而空斑试验管中只有16份（21%）CPE呈阳性（P小于0.01）。接种高滴度病毒制剂并在16小时进行IP染色的滚动培养和空斑试验管培养之间，在敏感性上没有差异。然而，当使用低滴度制剂时，接种后64小时，41份标本中有39份（95%）通过高速滚动法IP呈阳性，而空斑试验管中41份标本只有24份（58%）IP呈阳性（P小于0.01）。这些结果表明，对于通过IP染色检测单纯疱疹病毒，高速滚动法优于空斑试验管技术。
