Tenser R B
Infect Immun. 1978 Jul;21(1):281-5. doi: 10.1128/iai.21.1.281-285.1978.
By ultracentrifugation of 30 ml of highly dilute suspensions of herpes simplex virus (HSV) directly onto monolayer cultures grown in centrifuge tubes, infectivity was significantly greater than without centrifugation. Ultracentrifugation at 20,000 to 25,000 rpm (28,000 to 45,000 X g) for 1.5 to 2.3 h was utilized with good preservation of cultures. With low-speed centrifugation at 3,000 rpm (1,100 X g), infectivity was almost 10-fold greater than without centrifugation. With ultracentrifugal inoculation, infectivity was about 100-fold greater than without centrifugation. Ultracentrifugal inoculation permitted the detection of HSV at concentrations as low as 0.05 plaque-forming units per ml. Similarly, ultracentrifugal inoculation of cultures was almost 100-fold more sensitive a method of detecting infectious HSV than was pelleting HSV from dilute suspensions followed by resuspension and inoculation of cultures. Ultracentrifugal inoculation of cultures may permit the isolation of HSV in situations where virus cannot be detected by ordinary means and may prove applicable to the study of other viruses.
通过将30毫升高度稀释的单纯疱疹病毒（HSV）悬液直接超速离心到在离心管中生长的单层培养物上，其感染性显著高于未离心的情况。使用20,000至25,000转/分钟（28,000至45,000×g）的速度进行1.5至2.3小时的超速离心，培养物能得到良好保存。以3,000转/分钟（1,100×g）的低速离心时，感染性几乎比未离心时高10倍。采用超速离心接种时，感染性比未离心时高约100倍。超速离心接种能够检测到低至每毫升0.05个空斑形成单位浓度的HSV。同样，对培养物进行超速离心接种作为一种检测感染性HSV的方法，比从稀释悬液中沉淀HSV然后重悬并接种到培养物中要敏感近100倍。在无法通过常规方法检测到病毒的情况下，对培养物进行超速离心接种可能有助于分离HSV，并且可能适用于其他病毒的研究。
