Ma Tingting, Wang Yi, Chen Xiaoqian, Zhao Xiaoping
Zhejiang Chinese Medical University, Hangzhou 310053, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2017 Jan 25;46(1):66-73. doi: 10.3785/j.issn.1008-9292.2017.02.10.
To discover the nephroprotective substances from Huangkui capsule. The components of Huangkui capsule were isolated by preparative liquid chromatography, and the active components were screened by LC/MS and identified. The adriamycine-injured HK-2 cells were treated with various active components with different concentrations, and the malonaldehyde (MDA) content, adenosine triphosphate (ATP) level and mitochondrial oxygen consumption rate were measured to verify the protective activity of the compounds. Four active components in Huangkui capsule were identified to exert nephroprotective effects. Fifteen flavanoids from these four components were tentatively identified by LC/MS, and hyperin, myricetin, quercetin, rutin and isoquercetin were confirmed. Hyperin, myricetin quercetin and rutin showed dose-dependent protective effects on injured HK-2 cells. Espacially, hyperin significantly reduced MDA content, quercetin and rutin significantly increased ATP level, and myricetin significantly increased mitochondrial oxygen consumption rate. Hyperin, myricetin, querctein and rutin might be the potential nephroprotective compounds in Huangkui capsule, their effects may be related to the inhibition of lipid peroxidation and the alleviation of mitochondrial damage.
探索黄葵胶囊中的肾保护物质。采用制备液相色谱法分离黄葵胶囊的成分，通过液相色谱/质谱联用技术筛选并鉴定活性成分。用不同浓度的各种活性成分处理阿霉素损伤的HK-2细胞，检测丙二醛（MDA）含量、三磷酸腺苷（ATP）水平和线粒体氧消耗率，以验证化合物的保护活性。鉴定出黄葵胶囊中的四种活性成分具有肾保护作用。通过液相色谱/质谱联用技术初步鉴定了这四种成分中的15种黄酮类化合物，其中包括金丝桃苷、杨梅素、槲皮素、芦丁和异槲皮苷。金丝桃苷、杨梅素、槲皮素和芦丁对损伤的HK-2细胞具有剂量依赖性保护作用。特别是，金丝桃苷显著降低MDA含量，槲皮素和芦丁显著提高ATP水平，杨梅素显著提高线粒体氧消耗率。金丝桃苷、杨梅素、槲皮素和芦丁可能是黄葵胶囊中潜在的肾保护化合物，它们的作用可能与抑制脂质过氧化和减轻线粒体损伤有关。
