Holzbaur E L, Andrawis A, Tien M
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Biochem Biophys Res Commun. 1988 Sep 15;155(2):626-33. doi: 10.1016/s0006-291x(88)80541-2.
We have been investigating the structure and regulation of the lignin peroxidase genes from Phanerochaete chrysosporium. The gene for lignin peroxidase isozyme H8 was isolated and sequenced. The gene is split into nine exons and eight introns. The introns have consensus sequences both at the splice junctions and at an internal element which may be involved in the splicing mechanism. The promoter contains the eukaryotic consensus elements: a TATA box at -78 and a ACAAT box at -106. Transcription initiates downstream from these sequences as verified by S1 nuclease mapping. Induction of the genes for the lignin peroxidase isozymes during growth under nitrogen and under carbon limitation were compared by analyses of RNA and protein. The results indicate that the lignin peroxidase isozymes are differentially regulated in response to environmental stress.
我们一直在研究黄孢原毛平革菌木质素过氧化物酶基因的结构与调控。分离并测序了木质素过氧化物酶同工酶H8的基因。该基因被分割为9个外显子和8个内含子。内含子在剪接位点和一个可能参与剪接机制的内部元件处都有共有序列。启动子包含真核生物共有元件：在-78处有一个TATA盒，在-106处有一个ACAAT盒。如通过S1核酸酶作图所证实，转录起始于这些序列的下游。通过RNA和蛋白质分析比较了在氮限制和碳限制条件下生长期间木质素过氧化物酶同工酶基因的诱导情况。结果表明，木质素过氧化物酶同工酶对环境胁迫的反应存在差异调控。
